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Protein C Tag Antibody (A01774-100) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Protein C tagged protein transfected CHO cells. using THE™ Protein C Tag Antibody, mAb, Mouse (GenScript, A01774) and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007)The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Product Details
A01774-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2H12F4
Immunogen
Protein C tag peptide conjugated to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.GenScript THE™ Protein C (HPC4) Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.
Target Information
Protein C is a vitamin K-dependent plasma glycoprotein that is cleaved to its activated form by the thrombin-thrombomodulin complex. Protein C in its activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL. Mutations in the Protein C gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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