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Bethyl Laboratories
Pumilio 1 Polyclonal Antibody
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Pumilio 1 Antibody (A302-577A) in IP
Detection of human Pumilio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pumilio 1 antibody (Product # A302-577A) used for IP at 3 µg/mg lysate. Pumilio 1 was also immunoprecipitated by rabbit anti-Pumilio 1 antibody A302-576A, which recognizes an upstream epitope. For blotting immunoprecipitated Pumilio 1, A302-576A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A302-577A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 200 and 250 of Human Pumilio 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HsPUM; Pumilio homolog 1; pumilio RNA-binding family member 1; pumilio-1
Gene Aliases: HSPUM; KIAA0099; PUM1; PUMH; PUMH1; PUML1
UniProt ID: (Human) Q14671
Entrez Gene ID: (Human) 9698
spermatogenesis
adult locomotory behavior
posttranscriptional regulation of gene expression
posttranscriptional gene silencing
regulation of mRNA stability
stem cell differentiation
regulation of cell cycle
regulation of chromosome segregation
regulation of gene silencing by miRNA
mRNA destabilization
positive regulation of RIG-I signaling pathway
positive regulation of gene silencing by miRNA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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