Invitrogen

Qa-2 Monoclonal Antibody (69H1-9-9), FITC, eBioscience™

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Qa-2 Antibody (11-5996-82) in Flow

Staining of C57BL/6 splenocytes with 0.25 µg of Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (open histogram) or 0.25 µg of Anti-Mouse Qa-2 FITC (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Qa-2 Antibody — Figure 3 Thymic exosomes induce maturation of thymocytes. After three days in culture with thymic CD11c+ DCs and/or thymic exosomes (a), spleen exosomes (b), 100 nm latex beads (c), RNase treated thymic exosomes (d) or Proteinase K treated thymic exosomes (e); thymocytes were stained for surface expression of CD4+S1P1+Qa2+. After three days in culture with C57BL/6 or Balb/c thymic CD11c+ DCs, or C57BL/6 or Balb/c thymic exosomes, the C57BL/6 thymocytes were stained for surface expression of CD4+S1P1+Qa2+ (f). Left figure shows representative flow cytometry plots with indicated stimulations and surface markers. Right figure shows normalized flow cytometry data from at least three individual experiments. Data from exosome experiments are representative from at least three to five individual experiments. {TM}

Product Details

11-5996-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

69H1-9-9

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_465352

Product Specific Information

Description: The 69H1-9-9 monoclonal antibody reacts with the mouse Qa-2 molecule, a GPI-linked MHC class Ibeta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level is different depending on the laboratory mouse strain.

Applications Reported: The 69H1-9-9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 69H1-9-9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Qa-2 is a GPI-linked MHC class I beta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level varies between laboratory mouse strains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Imprint of Initial Education and Loss of Ly49C/I in Activated Natural Killer Cells of TAP1-KO and C57BL/6 Wildtype Mice.

Frontiers in immunology

Patil ND,Domingues O,Masquelier C,Theresine M,Schlienger O,Njinju Amin Asaba C,Thomas M,Seguin-Devaux C,Slevogt H,Ollert M,Zimmer J

11-5996-82 was used in Flow cytometry/Cell sorting to show that we completed our investigations with phenotypic studies of NK cells from these mice.

Tue Aug 02 00:00:00 UTC 2022

Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo.

Journal of cellular physiology

Thomas AM,Dong Y,Beskid NM,García AJ,Adams AB,Babensee JE

11-5996-82 was used in Flow Cytometry to show that expression of the costimulatory molecule CD86 was found to reliably increase when dendritic cells were exposed to hyperglycemia.

Mon Jun 01 00:00:00 UTC 2020

Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells.

Scientific reports

da Silva IL,Montero-Montero L,Martín-Villar E,Martin-Pérez J,Sainz B,Renart J,Toscano Simões R,Soares Veloso É,Salviano Teixeira C,de Oliveira MC,Ferreira E,Quintanilla M

11-5996 was used in Flow cytometry/Cell sorting to suggest an anti-malignant role for Qa-2 in breast cancer development.

Mon Jul 24 00:00:00 UTC 2017

NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.

The Journal of experimental medicine

Drennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D

11-5996 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells.

Mon Sep 19 00:00:00 UTC 2016

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

11-5996 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 UTC 2013

Spontaneous cardiac calcinosis in BALB/cByJ mice.

Comparative medicine

Glass AM,Coombs W,Taffet SM

11-5996 was used in Flow cytometry/Cell sorting to suggest that immune cells are actively recruited to calcinotic lesions and that myocyte gap junctions are altered near calcinotic lesions.

Fri Feb 01 00:00:00 UTC 2013

Dynamic expression of Qa-2 during acute graft rejection.

Molecular medicine (Cambridge, Mass.)

Lu N,Wang C,Yang X,Zhao S,Li X,Li X,Jiang H,Feng J,Zhang Y,Zou X

11-5996 was used in Flow cytometry/Cell sorting to investigate the dynamic expression of Qa-2 in skin allografts.

Mon Aug 01 00:00:00 UTC 2011

The role of tapasin in MHC class I protein trafficking in embryos and T cells.

Journal of reproductive immunology

Lampton PW,Goldstein CY,Warner CM

11-5996 was used in Flow cytometry/Cell sorting to study the role of chaperone molecules in regulating Qa-2, an MHC class Ib protein, expression in early mice embryos.

Sun Jun 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: H2Q7; Qa-2 antigen

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Gene Aliases: H2-Qa2; Ped; Qa-2

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Entrez Gene ID: (Mouse) 111458

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Performance Guarantee

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Qa-2 Monoclonal Antibody (69H1-9-9), FITC, eBioscience™

Qa-2 Antibody (11-5996-82) in Flow

Product Details

11-5996-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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