Invitrogen

RAB11A Recombinant Rabbit Monoclonal Antibody (3H18L5)

1 Published Figure

10 References

View all (14) RAB11A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Published Figures (1)

RAB11A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

RAB11A Antibody (700184) in ICC/IF

Immunofluorescence analysis of Ras-related protein Rab-11A was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAB11A Recombinant Rabbit Monoclonal Antibody (3H18L5) (Product # 700184) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antib... View More

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Product Details

700184

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2-5 µg

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Human, Mouse, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H18L5

Immunogen

A peptide corresponding to amino acids 179-194 of P62491.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532295

Product Specific Information

This antibody is predicted to react with bovine, chicken, equine, mouse, primate, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Rab11A is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11A mRNA has been detected in a variety of tissues including brain, testis, spleen, heart, and gastrointestinal mucosa. In the gastric fundus the Rab11A protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11A protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations. Recent studies have shown Rab11A is essential for resecretion of alpha-synuclein out of neuronal cells via endosome recycling and is required for exocytosis of discoidal/fusiform vesicles of bladder umbrella cells. Rab11A is also implicated in biogenesis of Birbeck granules and endosomal activation of the PI3K/AKT signaling pathway via G beta gamma trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spatial organization of lysosomal exocytosis relies on membrane tension gradients.

Proceedings of the National Academy of Sciences of the United States of America

Lachuer H,Le L,Lévêque-Fort S,Goud B,Schauer K

700184 was used in Immunocytochemistry to indicate that the spatial clustering of lysosomal exocytosis relies on membrane tension patterning controlled by the spatial organization of FAs.

Tue Feb 21 00:00:00 UTC 2023

TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes.

Nature communications

Wetzel L,Blanchard S,Rama S,Beier V,Kaufmann A,Wollert T

700184 was used in Immunocytochemistry-immunoflourescence to find that LC3C cooperates with lysosomal TECPR1 to promote the degradation of disease-related protein aggregates in neural stem cells.

Fri Jun 12 00:00:00 UTC 2020

Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog-Gli Is Required for Tracheoesophageal Separation.

Developmental cell

Nasr T,Mancini P,Rankin SA,Edwards NA,Agricola ZN,Kenny AP,Kinney JL,Daniels K,Vardanyan J,Han L,Trisno SL,Cha SW,Wells JM,Kofron MJ,Zorn AM

700184 was used in Immunohistochemistry to show that downstream of Gli the Foxf1+ splanchnic mesenchyme promotes medial constriction of the foregut at the boundary between the presumptive Sox2+ esophageal and Nkx2-1+ tracheal epithelium.

Mon Dec 16 00:00:00 UTC 2019

Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog-Gli Is Required for Tracheoesophageal Separation.

Developmental cell

Nasr T,Mancini P,Rankin SA,Edwards NA,Agricola ZN,Kenny AP,Kinney JL,Daniels K,Vardanyan J,Han L,Trisno SL,Cha SW,Wells JM,Kofron MJ,Zorn AM

Mon Dec 16 00:00:00 UTC 2019

Human DEF6 deficiency underlies an immunodeficiency syndrome with systemic autoimmunity and aberrant CTLA-4 homeostasis.

Nature communications

Serwas NK,Hoeger B,Ardy RC,Stulz SV,Sui Z,Memaran N,Meeths M,Krolo A,Yüce Petronczki Ö,Pfajfer L,Hou TZ,Halliday N,Santos-Valente E,Kalinichenko A,Kennedy A,Mace EM,Mukherjee M,Tesi B,Schrempf A,Pickl WF,Loizou JI,Kain R,Bidmon-Fliegenschnee B,Schickel JN,Glauzy S,Huemer J,Garncarz W,Salzer E,Pierides I,Bilic I,Thiel J,Priftakis P,Banerjee PP,Förster-Waldl E,Medgyesi D,Huber WD,Orange JS,Meffre E,Sansom DM,Bryceson YT,Altman A,Boztug K

700184 was used in Western Blotting to uncover DEF6 as player in immune homeostasis ensuring availability of the checkpoint protein CTLA-4 at T-cell surface, identifying a potential target for autoimmune and/or cancer therapy.

Mon Jul 15 00:00:00 UTC 2019

The extended cytoplasmic tail of the human B4GALNT2 is critical for its Golgi targeting and post-Golgi sorting.

The FEBS journal

Groux-Degroote S,Schulz C,Cogez V,Noël M,Portier L,Vicogne D,Solorzano C,Dall'Olio F,Steenackers A,Mortuaire M,Gonzalez-Pisfil M,Henry M,Foulquier F,Héliot L,Harduin-Lepers A

700184 was used in Immunocytochemistry-immunoflourescence to examine the subcellular distribution of the two B4GALNT2 protein isoforms in stably transfected colonic LS174T cells and in transiently transfected HeLa cells using fluorescence microscopy.

Sat Sep 01 00:00:00 UTC 2018

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Nature communications

He K,Ma X,Xu T,Li Y,Hodge A,Zhang Q,Torline J,Huang Y,Zhao J,Ling K,Hu J

700184 was used in Immunocytochemistry-immunoflourescence to reveal that tubulin polyglutamylation is a major contributor for cilia signalling and suggest a potential therapeutic strategy to correct signalling defects in ciliopathies.

Fri Aug 17 00:00:00 UTC 2018

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Nature communications

He K,Ma X,Xu T,Li Y,Hodge A,Zhang Q,Torline J,Huang Y,Zhao J,Ling K,Hu J

Fri Aug 17 00:00:00 UTC 2018

Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory.

eLife

Niu Y,Dai Z,Liu W,Zhang C,Yang Y,Guo Z,Li X,Xu C,Huang X,Wang Y,Shi YS,Liu JJ

700184 was used in immunohistochemistry to find that CNS-specific snx6-knockout mice exhibit deficits in spatial learning and memory, accompanied with loss of spines from distal dendrites of hippocampal CA1 pyramidal cells

Mon Jan 30 00:00:00 UTC 2017

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Nature communications

Bartuzi P,Billadeau DD,Favier R,Rong S,Dekker D,Fedoseienko A,Fieten H,Wijers M,Levels JH,Huijkman N,Kloosterhuis N,van der Molen H,Brufau G,Groen AK,Elliott AM,Kuivenhoven JA,Plecko B,Grangl G,McGaughran J,Horton JD,Burstein E,Hofker MH,van de Sluis B

700184 was used in western blot to analyze how WASH- and CCC-mediated endosomal sorting of LDLr is needed for regular clearance of circulating LDL

Fri Mar 11 00:00:00 UTC 2016

Exogenous α-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ronzitti G,Bucci G,Emanuele M,Leo D,Sotnikova TD,Mus LV,Soubrane CH,Dallas ML,Thalhammer A,Cingolani LA,Mochida S,Gainetdinov RR,Stephens GJ,Chieregatti E

700184 was used in western blot to test if alpha-synuclein modulates neurotransmitter release

Wed Aug 06 00:00:00 UTC 2014

Exogenous α-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ronzitti G,Bucci G,Emanuele M,Leo D,Sotnikova TD,Mus LV,Soubrane CH,Dallas ML,Thalhammer A,Cingolani LA,Mochida S,Gainetdinov RR,Stephens GJ,Chieregatti E

700184 was used in western blot to test if alpha-synuclein modulates neurotransmitter release

Wed Aug 06 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 24KG; RAB 11A, member oncogene family; Rab-11; Ras-related protein Rab-11A; YL8

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Gene Aliases: RAB11; RAB11A; YL8

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UniProt ID: (Human) P62491, (Rat) P62494, (Mouse) P62492

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Entrez Gene ID: (Human) 8766, (Rat) 81830, (Mouse) 53869

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Function(s)

GTPase activity protein binding GTP binding microtubule binding syntaxin binding myosin V binding GDP binding nucleotide binding

Process(es)

intracellular protein transport exocytosis mitotic metaphase plate congression metabolic process positive regulation of epithelial cell migration regulation of multivesicular body size positive regulation of G2/M transition of mitotic cell cycle vesicle-mediated transport astral microtubule organization neuron projection development melanosome transport Rab protein signal transduction multivesicular body assembly positive regulation of axon extension regulation of long-term neuronal synaptic plasticity regulation of protein transport establishment of vesicle localization regulation of vesicle-mediated transport establishment of protein localization to organelle protein localization to plasma membrane establishment of protein localization to membrane mitotic spindle assembly exosomal secretion cytokinesis renal water homeostasis small GTPase mediated signal transduction protein transport plasma membrane to endosome transport transport cell cycle

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RAB11A Recombinant Rabbit Monoclonal Antibody (3H18L5)

RAB11A Antibody (700184) in ICC/IF

Product Details

700184

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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