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Invitrogen
RAB11B Polyclonal Antibody
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RAB11B Antibody (PA1-775) in ICC/IF
Immunofluorescent analysis of Rab11B (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Rab11B rabbit polyclonal antibody (Product # PA1-775) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conju... View More
Product Details
PA1-775
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate
Published species
Human,
Sea urchin
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) G T R D D E Y D Y L F K V V L I C(17) of human Rab11.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2173598
Product Specific Information
PA1-775 detects Rab 11 from human and non-human primate samples. This antibody detects both A and B isoforms.
PA1-775 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
The PA1-775 immunizing peptide corresponds to amino acid residues 1-17 from human Rab 11. This sequence is completely conserved in human, mouse and rat and 94% conserved in C. elegans and amphibians. This peptide (Cat. # PEP-195) is available for use in neutralization and control experiments.
Target Information
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GTP-binding protein YPT3; RAB11B, member of RAS oncogene family; Ras-related protein Rab-11B
Gene Aliases: H-YPT3; RAB11B; YPT3
UniProt ID: (Human) Q15907
Entrez Gene ID: (Dog) 611619, (Human) 9230
receptor recycling
small GTPase mediated signal transduction
melanosome transport
transferrin transport
insulin secretion involved in cellular response to glucose stimulus
regulation of anion transport
constitutive secretory pathway
regulated exocytosis
cellular response to acidic pH
establishment of protein localization to membrane
cell-cell adhesion
retrograde transport, endosome to plasma membrane
regulation of protein localization to cell surface
regulation of endocytic recycling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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