Invitrogen

RAB11B Polyclonal Antibody

RAB11B Antibody in Immunocytochemistry (ICC/IF)

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RAB11B Antibody (PA5-31348) in ICC/IF

Immunofluorescence analysis of RAB11B was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB11B Polyclonal Antibody (Product # PA5-31348) at 1:500 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More

RAB11B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB11B Antibody in Immunohistochemistry (Frozen) (IHC (F))

RAB11B Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RAB11B Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-31348

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.67 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2548822

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, Mouse brain, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members.

eLife

Gemperle J,Harrison TS,Flett C,Adamson AD,Caswell PT

Published figure using RAB11B polyclonal antibody (Product # PA5-31348) in Western Blot

Thu Jun 16 00:00:00 UTC 2022

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.

Nature communications

Howe EN,Burnette MD,Justice ME,Schnepp PM,Hedrick V,Clancy JW,Guldner IH,Lamere AT,Li J,Aryal UK,D'Souza-Schorey C,Zartman JJ,Zhang S

PA5-31348 was used in Western Blot, Immunohistochemistry to combine time-course RNA-sequencing of BCBM development with a Drosophila melanogaster genetic screen, and identify Rab11b as a functional mediator of metastatic adaptation.

Mon Jun 15 00:00:00 UTC 2020

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.

Nature communications

Howe EN,Burnette MD,Justice ME,Schnepp PM,Hedrick V,Clancy JW,Guldner IH,Lamere AT,Li J,Aryal UK,D'Souza-Schorey C,Zartman JJ,Zhang S

Mon Jun 15 00:00:00 UTC 2020

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.

Nature communications

Howe EN,Burnette MD,Justice ME,Schnepp PM,Hedrick V,Clancy JW,Guldner IH,Lamere AT,Li J,Aryal UK,D'Souza-Schorey C,Zartman JJ,Zhang S

Mon Jun 15 00:00:00 UTC 2020

Rab11b-mediated integrin recycling promotes brain metastatic adaptation and outgrowth.

Nature communications

Howe EN,Burnette MD,Justice ME,Schnepp PM,Hedrick V,Clancy JW,Guldner IH,Lamere AT,Li J,Aryal UK,D'Souza-Schorey C,Zartman JJ,Zhang S

Mon Jun 15 00:00:00 UTC 2020

A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.

Oncotarget

Shimura M,Mizuma M,Takadate T,Katoh Y,Suzuki T,Iseki M,Hata T,Aoki S,Suzuki Y,Sakata N,Ohtsuka H,Hayashi H,Morikawa T,Nakagawa K,Motoi F,Naitoh T,Igarashi K,Sasano H,Unno M

Published figure using RAB11B polyclonal antibody (Product # PA5-31348) in Immunohistochemistry

Fri May 11 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: GTP-binding protein YPT3; RAB11B, member of RAS oncogene family; RAS oncogene family protein; Ras-related protein Rab-11B

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Gene Aliases: A730055L17Rik; H-YPT3; RAB11B; YPT3

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UniProt ID: (Human) Q15907, (Rat) O35509, (Mouse) P46638

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Entrez Gene ID: (Human) 9230, (Rat) 79434, (Mouse) 19326

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Function(s)

GTPase activity protein binding GTP binding GDP binding myosin V binding cadherin binding involved in cell-cell adhesion nucleotide binding

Process(es)

receptor recycling small GTPase mediated signal transduction melanosome transport transferrin transport insulin secretion involved in cellular response to glucose stimulus regulation of anion transport constitutive secretory pathway regulated exocytosis cellular response to acidic pH establishment of protein localization to membrane cell-cell adhesion retrograde transport, endosome to plasma membrane regulation of protein localization to cell surface regulation of endocytic recycling intracellular protein transport endocytosis Rab protein signal transduction transport protein transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB11B Polyclonal Antibody

RAB11B Antibody (PA5-31348) in ICC/IF

Product Details

PA5-31348

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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