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Invitrogen
RAB11B Monoclonal Antibody (6C5)
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FIGURE: 1 / 5
RAB11B Antibody (MA5-36243) in ICC/IF
Immunocytochemistry analysis of RAB11B in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in RAB11B monoclonal antibody (Product # MA5-36243) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36243
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6C5
Immunogen
A synthetic peptide corresponding to a sequence of human RAB11B (QKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884077
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 tissue, human Hela whole cell, human A549 whole cell, human placenta whole cell, human HepG2 whole cellhuman Caco-2 whole cellhuman THP-1 whole cellhuman Raji whole cell, rat brain tissue, rat lung whole cell, rat spleen whole cell, rat C6 whole cell, mouse brain whole cellmouse lung whole cellmouse spleen whole cellmouse Neuro-2a whole cellmouse RAW2467 whole cell. ICC/IF: MCF7 cell. Flow: Caco-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein YPT3; RAB11B, member of RAS oncogene family; RAS oncogene family protein; Ras-related protein Rab-11B
Gene Aliases: A730055L17Rik; H-YPT3; RAB11B; YPT3
UniProt ID: (Human) Q15907, (Rat) O35509, (Mouse) P46638
Entrez Gene ID: (Human) 9230, (Rat) 79434, (Mouse) 19326
receptor recycling
small GTPase mediated signal transduction
melanosome transport
transferrin transport
insulin secretion involved in cellular response to glucose stimulus
regulation of anion transport
constitutive secretory pathway
regulated exocytosis
cellular response to acidic pH
establishment of protein localization to membrane
cell-cell adhesion
retrograde transport, endosome to plasma membrane
regulation of protein localization to cell surface
regulation of endocytic recycling
intracellular protein transport
endocytosis
Rab protein signal transduction
transport
protein transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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