Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
RAB27A Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 4
RAB27A Antibody (PA5-47907) in ICC/IF
Immunocytochemical analysis of RAB27A was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-human/mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47907) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47907
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human RAB27A Ser135-Ala218
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608619
Product Specific Information
This antibody detects human, mouse, and rat RAB27A in Western blots and recombinant human RAB27A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human RAB27B is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ashen; GTP-binding protein Ram; low Mr GTP-binding protein; mutant Ras-related protein Rab-27A; Rab-27; Ram p25; Ras-related protein Rab-27A
Gene Aliases: 2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash; GS2; HsT18676; RAB27; RAB27A; RAM
UniProt ID: (Human) P51159, (Mouse) Q9ERI2, (Rat) P23640
Entrez Gene ID: (Human) 5873, (Mouse) 11891, (Rat) 50645
protein targeting
exocytosis
small GTPase mediated signal transduction
blood coagulation
positive regulation of gene expression
antigen processing and presentation
melanocyte differentiation
melanosome localization
melanosome transport
multivesicular body organization
cytotoxic T cell degranulation
natural killer cell degranulation
positive regulation of exocytosis
synaptic vesicle transport
positive regulation of phagocytosis
multivesicular body sorting pathway
complement-dependent cytotoxicity
positive regulation of regulated secretory pathway
positive regulation of reactive oxygen species biosynthetic process
positive regulation of constitutive secretory pathway
exosomal secretion
vesicle docking involved in exocytosis
protein secretion
protein transport
vesicle-mediated transport
Rab protein signal transduction
pigmentation
pigment granule localization
pigment granule transport
intracellular protein transport
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support