Proteintech

RAB3IP/Rabin8 Polyclonal Antibody

RAB3IP/Rabin8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

RAB3IP/Rabin8 Antibody (12321-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (Product # 12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAB3IP/Rabin8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB3IP/Rabin8 Antibody in Western Blot (WB)

RAB3IP/Rabin8 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12321-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAB3IP/Rabin8 Fusion Protein Ag2974 (21-254 aa encoded by BC015548)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GKIDVLQAE VAALKTLVLS SSPTSPTQEP LPGGKTPFKK GHTRNKSTSS AMSGSHQDLS VIQPIVKDCK EADLSLYNEF RLWKDEPTMD RTCPFLDKIY QEDIFPCLTF SKSELASAVL EAVENNTLSI EPVGLQPIRF VKASAVECGG PKKCALTGQS KSCKHRIKLG DSSNYYYISP FCRYRITSVC NFFTYIRYIQ QGLVKQQDVD QMFWEVMQLR KEMSLAKLGY FKEEL (21-254 aa encoded by B C015548)

Target Information

Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protein quality control machinery supports primary ciliogenesis by eliminating GDP-bound Rab8-family GTPases.

iScience

Takahashi T,Shirai J,Matsuda M,Nakanaga S,Matsushita S,Wakita K,Hayashishita M,Suzuki R,Noguchi A,Yokota N,Kawahara H

Fri May 19 00:00:00 UTC 2023

Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.

Small GTPases

Tong SJ,Wall AA,Hung Y,Luo L,Stow JL

Fri Jan 01 00:00:00 UTC 2021

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

The EMBO journal

Miyamoto T,Hosoba K,Itabashi T,Iwane AH,Akutsu SN,Ochiai H,Saito Y,Yamamoto T,Matsuura S

Wed Jun 17 00:00:00 UTC 2020

Cytoplasmic control of Rab family small GTPases through BAG6.

EMBO reports

Takahashi T,Minami S,Tsuchiya Y,Tajima K,Sakai N,Suga K,Hisanaga SI,Ohbayashi N,Fukuda M,Kawahara H

Mon Apr 01 00:00:00 UTC 2019

The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

The Journal of biological chemistry

Cuenca A,Insinna C,Zhao H,John P,Weiss MA,Lu Q,Walia V,Specht S,Manivannan S,Stauffer J,Peden AA,Westlake CJ

Fri Oct 18 00:00:00 UTC 2019

Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.

Developmental cell

Walia V,Cuenca A,Vetter M,Insinna C,Perera S,Lu Q,Ritt DA,Semler E,Specht S,Stauffer J,Morrison DK,Lorentzen E,Westlake CJ

Mon Jul 22 00:00:00 UTC 2019

Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis.

Developmental cell

Walia V,Cuenca A,Vetter M,Insinna C,Perera S,Lu Q,Ritt DA,Semler E,Specht S,Stauffer J,Morrison DK,Lorentzen E,Westlake CJ

Mon Jul 22 00:00:00 UTC 2019

Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.

The Journal of biological chemistry

Tao T,Sun J,Peng Y,Li Y,Wang P,Chen X,Zhao W,Zheng YY,Wei L,Wang W,Zhou Y,Liu J,Shi YS,Zhu MS

Fri Jul 12 00:00:00 UTC 2019

Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.

The Journal of biological chemistry

Tao T,Sun J,Peng Y,Li Y,Wang P,Chen X,Zhao W,Zheng YY,Wei L,Wang W,Zhou Y,Liu J,Shi YS,Zhu MS

Fri Jul 12 00:00:00 UTC 2019

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Proceedings of the National Academy of Sciences of the United States of America

Chen L,Chen Q,Xie B,Quan C,Sheng Y,Zhu S,Rong P,Zhou S,Sakamoto K,MacKintosh C,Wang HY,Chen S

Tue Jun 28 00:00:00 UTC 2016

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Proceedings of the National Academy of Sciences of the United States of America

Chen L,Chen Q,Xie B,Quan C,Sheng Y,Zhu S,Rong P,Zhou S,Sakamoto K,MacKintosh C,Wang HY,Chen S

Tue Jun 28 00:00:00 UTC 2016

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Proceedings of the National Academy of Sciences of the United States of America

Chen L,Chen Q,Xie B,Quan C,Sheng Y,Zhu S,Rong P,Zhou S,Sakamoto K,MacKintosh C,Wang HY,Chen S

Tue Jun 28 00:00:00 UTC 2016

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Proceedings of the National Academy of Sciences of the United States of America

Chen L,Chen Q,Xie B,Quan C,Sheng Y,Zhu S,Rong P,Zhou S,Sakamoto K,MacKintosh C,Wang HY,Chen S

Tue Jun 28 00:00:00 UTC 2016

Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.

The EMBO journal

Lai YC,Kondapalli C,Lehneck R,Procter JB,Dill BD,Woodroof HI,Gourlay R,Peggie M,Macartney TJ,Corti O,Corvol JC,Campbell DG,Itzen A,Trost M,Muqit MM

Thu Nov 12 00:00:00 UTC 2015

Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.

The EMBO journal

Lai YC,Kondapalli C,Lehneck R,Procter JB,Dill BD,Woodroof HI,Gourlay R,Peggie M,Macartney TJ,Corti O,Corvol JC,Campbell DG,Itzen A,Trost M,Muqit MM

Thu Nov 12 00:00:00 UTC 2015

GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.

PLoS biology

Zhang B,Zhang T,Wang G,Wang G,Chi W,Jiang Q,Zhang C

Wed Apr 01 00:00:00 UTC 2015

GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.

PLoS biology

Zhang B,Zhang T,Wang G,Wang G,Chi W,Jiang Q,Zhang C

Wed Apr 01 00:00:00 UTC 2015

GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.

PLoS biology

Zhang B,Zhang T,Wang G,Wang G,Chi W,Jiang Q,Zhang C

Wed Apr 01 00:00:00 UTC 2015

GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.

PLoS biology

Zhang B,Zhang T,Wang G,Wang G,Chi W,Jiang Q,Zhang C

Wed Apr 01 00:00:00 UTC 2015

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

RAB8B is required for activity and caveolar endocytosis of LRP6.

Cell reports

Demir K,Kirsch N,Beretta CA,Erdmann G,Ingelfinger D,Moro E,Argenton F,Carl M,Niehrs C,Boutros M

Thu Sep 26 00:00:00 UTC 2013

RAB8B is required for activity and caveolar endocytosis of LRP6.

Cell reports

Demir K,Kirsch N,Beretta CA,Erdmann G,Ingelfinger D,Moro E,Argenton F,Carl M,Niehrs C,Boutros M

Thu Sep 26 00:00:00 UTC 2013

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Neuron

Ultanir SK,Hertz NT,Li G,Ge WP,Burlingame AL,Pleasure SJ,Shokat KM,Jan LY,Jan YN

Thu Mar 22 00:00:00 UTC 2012

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Neuron

Ultanir SK,Hertz NT,Li G,Ge WP,Burlingame AL,Pleasure SJ,Shokat KM,Jan LY,Jan YN

Thu Mar 22 00:00:00 UTC 2012

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Neuron

Ultanir SK,Hertz NT,Li G,Ge WP,Burlingame AL,Pleasure SJ,Shokat KM,Jan LY,Jan YN

Thu Mar 22 00:00:00 UTC 2012

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Neuron

Ultanir SK,Hertz NT,Li G,Ge WP,Burlingame AL,Pleasure SJ,Shokat KM,Jan LY,Jan YN

Thu Mar 22 00:00:00 UTC 2012

Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.

Acta naturae

Parkhitko CA,Favorova CO,Henske EP

Fri Jul 01 00:00:00 UTC 2011

Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.

Acta naturae

Parkhitko CA,Favorova CO,Henske EP

Fri Jul 01 00:00:00 UTC 2011

Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.

Acta naturae

Parkhitko CA,Favorova CO,Henske EP

Fri Jul 01 00:00:00 UTC 2011

Rabin8 Protein Interacts with GTPase Rheb and Inhibits Phosphorylation of Ser235/Ser236 in Small Ribosomal Subunit Protein S6.

Acta naturae

Parkhitko CA,Favorova CO,Henske EP

Fri Jul 01 00:00:00 UTC 2011

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: gene trap PAT 12; Rab-3A-interacting protein; RAB3A-interacting protein; RAB3IP; rab3ip {ECO:0000250|UniProtKB:Q96QF0}; rabin 3; Rabin-3; Rabin8; SSX2 interacting protein; SSX2-interacting protein

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Gene Aliases: B230311A06; Gtpat12; RAB3IP; RABIN3; RABIN8

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UniProt ID: (Human) Q96QF0, (Rat) Q62739, (Mouse) Q68EF0

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Entrez Gene ID: (Human) 117177, (Rat) 29885, (Mouse) 216363

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Function(s)

protein binding Rab guanyl-nucleotide exchange factor activity GTPase binding guanyl-nucleotide exchange factor activity

Process(es)

protein targeting to membrane positive regulation of GTPase activity protein transport protein localization to organelle cilium assembly transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB3IP/Rabin8 Polyclonal Antibody

RAB3IP/Rabin8 Antibody (12321-1-AP) in ICC/IF

Product Details

12321-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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