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Invitrogen
RAB3d Polyclonal Antibody
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FIGURE: 1 / 1
RAB3d Antibody (PA5-87863) in WB
Western blot analysis of RAB3d in extracts of various cell lines. Samples were incubated with RAB3d Polyclonal antibody (Product # PA5-87863) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-87863
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human RAB3D (NP_0042741)
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804465
Product Specific Information
Immunogen sequence: MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC; Positive Samples: HepG2, U-87MG, MCF7, SKOV3, Mouse lung, Mouse pancreas; Cellular Location: Cell membrane, Cytoplasmic side, Lipid-anchor
Target Information
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glioblastoma overexpressed; Rab3D upregulated with myeloid differentiation; Ras-related protein Rab-3D
Gene Aliases: C130057E11Rik; D2-2; GOV; RAB16; RAB3D; RAD3D
UniProt ID: (Human) O95716, (Mouse) P35276
Entrez Gene ID: (Human) 9545, (Mouse) 19340
exocytosis
small GTPase mediated signal transduction
protein transport
peptidyl-cysteine methylation
bone resorption
positive regulation of regulated secretory pathway
transport
intracellular protein transport
vesicle docking involved in exocytosis
protein secretion
synaptic vesicle exocytosis
regulation of exocytosis
Rab protein signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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