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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RAB5 Antibodies

          Proteintech

          RAB5A Polyclonal Antibody

          37 References
          View all (22) RAB5 antibodies

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          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite RAB5A Polyclonal Antibody

          • Antibody Testing Data (11)
          RAB5A Antibody in Immunocytochemistry (ICC/IF)
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          RAB5A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          RAB5A Antibody (11947-1-AP) in ICC/IF

          Immunofluorescent analysis of ( -20°CMethanol) fixed NIH/3T3 cells using 11947-1-AP (RAB5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RAB5A Antibody in Immunocytochemistry (ICC/IF)
          RAB5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RAB5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RAB5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RAB5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RAB5A Antibody in Western Blot (WB)
          RAB5A Antibody in Western Blot (WB)
          RAB5A Antibody in Western Blot (WB)
          RAB5A Antibody in Western Blot (WB)
          RAB5A Antibody in Western Blot (WB)
          RAB5A Antibody in Immunoprecipitation (IP)

          Product Details

          11947-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 19 publications 19 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 11 publications 11 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          RAB5A Fusion Protein Ag2549 (1-215 aa encoded by BC001267)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.23 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MASRGATRP NGPNTGNKIC QFKLVLLGES AVGKSSLVLR FVKGQFHEFQ ESTIGAAFLT QTVCLDDTTV KFEIWDTAGQ ERYHSLAPMY YRGAQAAIVV YDITNEESFA RAKNWVKELQ RQASPNIVIA LSGNKADLAN KRAVDFQEAQ SYADDNSLLF METSAKTSMN VNEIFMAIAK KLPKNEPQNP GANSARGRGV DLTEPTQPTR NQCCSN (1-215 aa encoded by B C001267)

          Target Information

          RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A; Small GTP-binding protein rab5

          View more View less

          Gene Aliases: 2410015H04Rik; AI663973; AU021172; nnyRab5a; RAB5; RAB5A

          View more View less

          UniProt ID: (Human) P20339, (Mouse) Q9CQD1, (Rat) M0RC99

          View more View less

          Entrez Gene ID: (Human) 5868, (Mouse) 271457, (Rat) 64633

          View more View less

          Function(s)
          GTPase activity protein binding GTP binding GDP binding nucleotide binding guanyl nucleotide binding GDP-dissociation inhibitor binding
          Process(es)
          endocytosis phagocytosis small GTPase mediated signal transduction blood coagulation protein transport regulation of endocytosis synaptic vesicle recycling early endosome to late endosome transport positive regulation of exocytosis regulation of endosome size regulation of filopodium assembly receptor internalization involved in canonical Wnt signaling pathway regulation of synaptic vesicle exocytosis regulation of autophagosome assembly transport intracellular protein transport endosome organization positive regulation of smooth muscle cell migration Rab protein signal transduction regulation of long-term neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation vesicle-mediated transport
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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