Invitrogen

RAB5A Monoclonal Antibody (2E8B11), eBioscience™

RAB5A Antibody in Immunocytochemistry (ICC/IF)

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RAB5A Antibody (14-9711-82) in ICC/IF

For immunofluorescence analysis, HeLa cells were fixed for detection of endogenous Rab5a using Anti-Rab5a Monoclonal Antibody (Product # 14-9711-82, 1:100 dilution) and labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000). Panel a) shows representative cells that were stained for detection of Rab5a protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin ... View More

RAB5A Antibody in Immunoprecipitation (IP)

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RAB5A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Rab5a siRNA (Silencer® select Product # s11679; s11680) and decrease in signal intensity was observed in western blot application using Anti-Rab5a Monoclonal Antibody. Western blot analysis (Fig. a) was performed using whole cell extracts from cells transfected with Rab5a siRNA (lane 3), non-specific scrambled siRNA (lane 2) or left untransfected (lane 1). The blot was probed with Anti-Rab5a Monoclonal Antibody (Product # 14-9711-82, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in the histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that the antibody is specific to Rab5a. {KD}

Product Details

14-9711-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2E8B11

Immunogen

Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2884043

Product Specific Information

Description: This 2E8B11 monoclonal antibody recognizes human Rab5a, which is a GTPase involved in intracellular membrane trafficking. Rab5a is reported to have ubiquitous expression.

Applications Reported: The 2E8B11 clone has been reported for use in intracellular flow cytometry, western blot, ELISA, immunocytochemistry of cells and immunohistochemistry of tissue.

Applications Tested: This 2E8B11 antibody has been tested by western blot in multiple human cell lines.
Applications Tested: This 2E8B11 antibody has been tested by immunocytochemical staining of fixed and permeabilized HeLa cells.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A

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Gene Aliases: RAB5; RAB5A

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UniProt ID: (Human) P20339

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Entrez Gene ID: (Human) 5868

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Function(s)

GTPase activity protein binding GTP binding GDP binding

Process(es)

endocytosis phagocytosis small GTPase mediated signal transduction blood coagulation protein transport regulation of endocytosis synaptic vesicle recycling early endosome to late endosome transport positive regulation of exocytosis regulation of endosome size regulation of filopodium assembly receptor internalization involved in canonical Wnt signaling pathway regulation of synaptic vesicle exocytosis regulation of autophagosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB5A Monoclonal Antibody (2E8B11), eBioscience™

RAB5A Antibody (14-9711-82) in ICC/IF

Product Details

14-9711-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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