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RAB7 Polyclonal Antibody

RAB7 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

RAB7 Antibody (OSR00306W-100UL) in ICC/IF

Human Melanoma cell line C32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µL of Rabbit antibody to internal region of Ras-related protein Rab-7a (RAB7A, RAB7): whole serum (OSR00306W) diluted 1:100 in the blocking buffer for 30 minutes. Welles were t... View More

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Product Details

OSR00306W-100UL

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:300-1:2,000

Immunocytochemistry (ICC/IF)

1:100

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 160-205 of human RAB7 conjugated to blue carrier protein has been used as the antigen

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in mouse and xenopus.

Specificity of this antibody: RAB7.

Target Information

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Complete sequencing and characterization of 21,243 full-length human cDNAs.

Nature genetics

Ota T,Suzuki Y,Nishikawa T,Otsuki T,Sugiyama T,Irie R,Wakamatsu A,Hayashi K,Sato H,Nagai K,Kimura K,Makita H,Sekine M,Obayashi M,Nishi T,Shibahara T,Tanaka T,Ishii S,Yamamoto J,Saito K,Kawai Y,Isono Y,Nakamura Y,Nagahari K,Murakami K,Yasuda T,Iwayanagi T,Wagatsuma M,Shiratori A,Sudo H,Hosoiri T,Kaku Y,Kodaira H,Kondo H,Sugawara M,Takahashi M,Kanda K,Yokoi T,Furuya T,Kikkawa E,Omura Y,Abe K,Kamihara K,Katsuta N,Sato K,Tanikawa M,Yamazaki M,Ninomiya K,Ishibashi T,Yamashita H,Murakawa K,Fujimori K,Tanai H,Kimata M,Watanabe M,Hiraoka S,Chiba Y,Ishida S,Ono Y,Takiguchi S,Watanabe S,Yosida M,Hotuta T,Kusano J,Kanehori K,Takahashi-Fujii A,Hara H,Tanase TO,Nomura Y,Togiya S,Komai F,Hara R,Takeuchi K,Arita M,Imose N,Musashino K,Yuuki H,Oshima A,Sasaki N,Aotsuka S,Yoshikawa Y,Matsunawa H,Ichihara T,Shiohata N,Sano S,Moriya S,Momiyama H,Satoh N,Takami S,Terashima Y,Suzuki O,Nakagawa S,Senoh A,Mizoguchi H,Goto Y,Shimizu F,Wakebe H,Hishigaki H,Watanabe T,Sugiyama A,Takemoto M,Kawakami B,Yamazaki M,Watanabe K,Kumagai A,Itakura S,Fukuzumi Y,Fujimori Y,Komiyama M,Tashiro H,Tanigami A,Fujiwara T,Ono T,Yamada K,Fujii Y,Ozaki K,Hirao M,Ohmori Y,Kawabata A,Hikiji T,Kobatake N,Inagaki H,Ikema Y,Okamoto S,Okitani R,Kawakami T,Noguchi S,Itoh T,Shigeta K,Senba T,Matsumura K,Nakajima Y,Mizuno T,Morinaga M,Sasaki M,Togashi T,Oyama M,Hata H,Watanabe M,Komatsu T,Mizushima-Sugano J,Satoh T,Shirai Y,Takahashi Y,Nakagawa K,Okumura K,Nagase T,Nomura N,Kikuchi H,Masuho Y,Yamashita R,Nakai K,Yada T,Nakamura Y,Ohara O,Isogai T,Sugano S

Thu Jan 01 00:00:00 UTC 2004

Molecular cloning and expression analysis of the human Rab7 GTP-ase complementary deoxyribonucleic acid.

Biochemical and biophysical research communications

Vitelli R,Chiariello M,Lattero D,Bruni CB,Bucci C

Tue Dec 24 00:00:00 UTC 1996

Bioinformatics

Protein Aliases: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein Rab-7a

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Gene Aliases: PRO2706; RAB7; RAB7A

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UniProt ID: (Human) P51149, (Mouse) P51150

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Entrez Gene ID: (Human) 7879, (Mouse) 19349

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Function(s)

GTPase activity protein binding GTP binding GDP binding Rac GTPase binding nucleotide binding

Process(es)

protein targeting to lysosome transport intracellular protein transport epidermal growth factor catabolic process small GTPase mediated signal transduction endosome to lysosome transport protein transport viral release from host cell protein to membrane docking Rab protein signal transduction retrograde transport, endosome to Golgi early endosome to late endosome transport bone resorption positive regulation of protein catabolic process positive regulation of viral process phagosome acidification phagosome-lysosome fusion positive regulation of exosomal secretion regulation of autophagosome assembly endocytosis antigen processing and presentation of exogenous peptide antigen via MHC class II lipophagy phagosome maturation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB7 Polyclonal Antibody

RAB7 Antibody (OSR00306W-100UL) in ICC/IF

Product Details

OSR00306W-100UL

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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