Invitrogen

RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03)

RAB7A Antibody in Immunocytochemistry (ICC/IF)

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RAB7A Antibody (MA5-32340) in ICC/IF

Immunofluorescence of RAB7A was performed using HeLa WT and RAB7A KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stained ... View More

RAB7A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAB7A Antibody — Antibody specificity was demonstrated by knockout (KO) of target protein. Loss of signal in immunofluorescence was observed for target protein in RAB7A KO cell line using RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03) (Product # MA5-32340). Parental cells were labelled with CellTracker™ Green CMFDA Dye (Product # C2925) and DDX1 KO cells with CellTracker™ Deep Red Dye (Product # C34565). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockdown/knockdown validation. {KO}

Product Details

MA5-32340

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN202-03

Immunogen

Synthetic peptide within Human RAB7 aa 158-207

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809621

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein BRL-RAS; Ras-related protein p23; Ras-related protein Rab-7a

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Gene Aliases: PRO2706; RAB7; RAB7A

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UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527

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Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448

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Function(s)

GTPase activity protein binding GTP binding GDP binding Rac GTPase binding nucleotide binding

Process(es)

protein targeting to lysosome transport intracellular protein transport epidermal growth factor catabolic process small GTPase mediated signal transduction endosome to lysosome transport protein transport viral release from host cell protein to membrane docking Rab protein signal transduction retrograde transport, endosome to Golgi early endosome to late endosome transport bone resorption positive regulation of protein catabolic process positive regulation of viral process phagosome acidification phagosome-lysosome fusion positive regulation of exosomal secretion regulation of autophagosome assembly endocytosis antigen processing and presentation of exogenous peptide antigen via MHC class II lipophagy phagosome maturation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB7A Recombinant Rabbit Monoclonal Antibody (SN202-03)

RAB7A Antibody (MA5-32340) in ICC/IF

Product Details

MA5-32340

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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