Proteintech

RAB8A Polyclonal Antibody

RAB8A Antibody in Immunocytochemistry (ICC/IF)

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RAB8A Antibody (55296-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 55296-1-AP (RAB8A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RAB8A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB8A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

55296-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 10 publications 10 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to RAB8A.

Target Information

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells.

Cells

Nakamura NK,Tokunaga DS,Ha HY,Polgar N

Sat Aug 06 00:00:00 UTC 2022

Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.

Cancer letters

Liu Y,Zhang Z,Gao X,Ma Q,Yu Z,Huang S

Wed Jun 01 00:00:00 UTC 2022

Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.

Food chemistry

Hu S,Hu C,Luo L,Zhang H,Zhao S,Liu Z,Zeng L

Tue Nov 15 00:00:00 UTC 2022

Mannan-Binding Lectin via Interaction With Cell Surface Calreticulin Promotes Senescence of Activated Hepatic Stellate Cells to Limit Liver Fibrosis Progression.

Cellular and molecular gastroenterology and hepatology

Luo J,Li L,Chang B,Zhu Z,Deng F,Hu M,Yu Y,Lu X,Chen Z,Zuo D,Zhou J

Mon Jun 20 00:00:00 UTC 2022

Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes.

Journal of Cancer

Wang Y,Li Y,Zhou H,Qian X,Hu Y

Wed Mar 24 00:00:00 UTC 2021

Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.

Cell death & disease

Zhuravleva V,Vaz-Silva J,Zhu M,Gomes P,Silva JM,Sousa N,Sotiropoulos I,Waites CL

Wed Dec 08 00:00:00 UTC 2021

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Cell research

Liu M,Zhang W,Li M,Feng J,Kuang W,Chen X,Yang F,Sun Q,Xu Z,Hua J,Yang C,Liu W,Shu Q,Yang Y,Zhou T,Xie S

Mon Nov 01 00:00:00 UTC 2021

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Cell research

Liu M,Zhang W,Li M,Feng J,Kuang W,Chen X,Yang F,Sun Q,Xu Z,Hua J,Yang C,Liu W,Shu Q,Yang Y,Zhou T,Xie S

Mon Nov 01 00:00:00 UTC 2021

Legionella remodels the plasma membrane-derived vacuole by utilizing exocyst components as tethers.

The Journal of cell biology

Arasaki K,Kimura H,Tagaya M,Roy CR

Mon Nov 05 00:00:00 UTC 2018

Legionella remodels the plasma membrane-derived vacuole by utilizing exocyst components as tethers.

The Journal of cell biology

Arasaki K,Kimura H,Tagaya M,Roy CR

Mon Nov 05 00:00:00 UTC 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19.

Journal of cell science

Ampah KK,Greaves J,Shun-Shion AS,Asnawi AW,Lidster JA,Chamberlain LH,Collins MO,Peden AA

Mon Oct 22 00:00:00 UTC 2018

S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19.

Journal of cell science

Ampah KK,Greaves J,Shun-Shion AS,Asnawi AW,Lidster JA,Chamberlain LH,Collins MO,Peden AA

Mon Oct 22 00:00:00 UTC 2018

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Human molecular genetics

Kim MJ,Jeon S,Burbulla LF,Krainc D

Fri Jun 01 00:00:00 UTC 2018

The Parkinson's disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking.

Human molecular genetics

Kim MJ,Deng HX,Wong YC,Siddique T,Krainc D

Wed Feb 15 00:00:00 UTC 2017

The Parkinson's disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking.

Human molecular genetics

Kim MJ,Deng HX,Wong YC,Siddique T,Krainc D

Wed Feb 15 00:00:00 UTC 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 UTC 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 UTC 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 UTC 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 UTC 2017

Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.

Circulation research

Barry DM,Koo Y,Norden PR,Wylie LA,Xu K,Wichaidit C,Azizoglu DB,Zheng Y,Cobb MH,Davis GE,Cleaver O

Fri Sep 16 00:00:00 UTC 2016

Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.

Circulation research

Barry DM,Koo Y,Norden PR,Wylie LA,Xu K,Wichaidit C,Azizoglu DB,Zheng Y,Cobb MH,Davis GE,Cleaver O

Fri Sep 16 00:00:00 UTC 2016

The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.

Scientific reports

Yu F,Sharma S,Skowronek A,Erdmann KS

Fri Oct 21 00:00:00 UTC 2016

The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.

Scientific reports

Yu F,Sharma S,Skowronek A,Erdmann KS

Fri Oct 21 00:00:00 UTC 2016

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

The Journal of cell biology

Burke MC,Li FQ,Cyge B,Arashiro T,Brechbuhl HM,Chen X,Siller SS,Weiss MA,O'Connell CB,Love D,Westlake CJ,Reynolds SD,Kuriyama R,Takemaru K

Mon Oct 13 00:00:00 UTC 2014

RAB8A a new biomarker for endometrial cancer?

World journal of surgical oncology

Bie Y,Zhang Z

Thu Dec 04 00:00:00 UTC 2014

RAB8A a new biomarker for endometrial cancer?

World journal of surgical oncology

Bie Y,Zhang Z

Thu Dec 04 00:00:00 UTC 2014

Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.

Nature communications

Veleri S,Manjunath SH,Fariss RN,May-Simera H,Brooks M,Foskett TA,Gao C,Longo TA,Liu P,Nagashima K,Rachel RA,Li T,Dong L,Swaroop A

Fri Jun 20 00:00:00 UTC 2014

Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.

Nature communications

Veleri S,Manjunath SH,Fariss RN,May-Simera H,Brooks M,Foskett TA,Gao C,Longo TA,Liu P,Nagashima K,Rachel RA,Li T,Dong L,Swaroop A

Fri Jun 20 00:00:00 UTC 2014

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Proceedings of the National Academy of Sciences of the United States of America

Humbert MC,Weihbrecht K,Searby CC,Li Y,Pope RM,Sheffield VC,Seo S

Tue Nov 27 00:00:00 UTC 2012

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Proceedings of the National Academy of Sciences of the United States of America

Humbert MC,Weihbrecht K,Searby CC,Li Y,Pope RM,Sheffield VC,Seo S

Tue Nov 27 00:00:00 UTC 2012

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

A molecular network for de novo generation of the apical surface and lumen.

Nature cell biology

Bryant DM,Datta A,Rodríguez-Fraticelli AE,Peränen J,Martín-Belmonte F,Mostov KE

Mon Nov 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: cell line NK14 derived transforming oncogene; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; ras-associated protein RAB8; Ras-related protein Rab-8A

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Gene Aliases: AA409338; MEL; RAB8; RAB8A

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UniProt ID: (Human) P61006, (Mouse) P55258, (Rat) P35280

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Entrez Gene ID: (Human) 4218, (Mouse) 17274, (Rat) 117103

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Function(s)

GTPase activity protein binding GTP binding Rab GTPase binding GDP binding protein kinase binding myosin V binding nucleotide binding

Process(es)

G2/M transition of mitotic cell cycle vesicle docking involved in exocytosis small GTPase mediated signal transduction axonogenesis protein secretion cellular response to insulin stimulus cilium assembly regulation of long-term neuronal synaptic plasticity Golgi vesicle fusion to target membrane regulation of protein transport membrane organization protein localization to plasma membrane transport intracellular protein transport metabolic process protein transport synaptic vesicle exocytosis regulation of exocytosis cell projection organization Rab protein signal transduction regulation of protein localization glucose homeostasis positive regulation of glucose import

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB8A Polyclonal Antibody

RAB8A Antibody (55296-1-AP) in ICC/IF

Product Details

55296-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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