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Invitrogen
RAB8A Recombinant Rabbit Monoclonal Antibody (JE64-79)
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FIGURE: 1 / 8
RAB8A Antibody (MA5-49140) in ICC/IF
Immunofluorescence analysis of RAB8A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB8A Rabbit Recombinant Monoclonal Antibody (JE64-79) (Product # MA5-49140 ) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More
Product Details
MA5-49140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE64-79
Immunogen
Synthetic peptide within human rab8a aa 151-200/207.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074798
Product Specific Information
Sequence Similarities: 100% Mouse/Rat
Tissue Specificity: Low tissue specificity
Positive Control: Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate
Subcellular location: Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody.
Target Information
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell line NK14 derived transforming oncogene; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; ras-associated protein RAB8; Ras-related protein Rab-8A
Gene Aliases: AA409338; MEL; RAB8; RAB8A
UniProt ID: (Human) P61006, (Mouse) P55258, (Rat) P35280
Entrez Gene ID: (Human) 4218, (Mouse) 17274, (Rat) 117103
G2/M transition of mitotic cell cycle
vesicle docking involved in exocytosis
small GTPase mediated signal transduction
axonogenesis
protein secretion
cellular response to insulin stimulus
cilium assembly
regulation of long-term neuronal synaptic plasticity
Golgi vesicle fusion to target membrane
regulation of protein transport
membrane organization
protein localization to plasma membrane
transport
intracellular protein transport
metabolic process
protein transport
synaptic vesicle exocytosis
regulation of exocytosis
cell projection organization
Rab protein signal transduction
regulation of protein localization
glucose homeostasis
positive regulation of glucose import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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