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Invitrogen
RAD3 Polyclonal Antibody
Product Details
PA5-32174
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of Saccharomyces cerevisiae S288c RAD3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549647
Product Specific Information
Recommended positive controls: Yeast lysate.
Predicted reactivity: Yeast (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP-dependent DNA helicase involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Necessary for excision of pyrimidine dimers. Also unwinds DNA/RNA duplexes. Plays an essential role in the cell viability. Involved in the maintenance of the fidelity of DNA replication. Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA 5'-3' helicase RAD3; DNA repair helicase RAD3; General transcription and DNA repair factor IIH helicase subunit XPD/RAD3; Rad3p; RNA polymerase II transcription factor B subunit RAD3; TFB subunit RAD3; TFIIH subunit XPD
Gene Aliases: RAD3; REM1; YER171W
UniProt ID: (Baker's yeast) P06839
Entrez Gene ID: (Baker's yeast) 856918
nucleotide binding
transcription factor activity, core RNA polymerase binding
nucleic acid binding
DNA binding
damaged DNA binding
ATP-dependent DNA helicase activity
helicase activity
ATP binding
ATP-dependent helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ATP-dependent 5'-3' DNA helicase activity
metal ion binding
iron-sulfur cluster binding
4 iron, 4 sulfur cluster binding
regulation of mitotic recombination
nucleobase-containing compound metabolic process
DNA repair
nucleotide-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
regulation of transposition, RNA-mediated
nucleotide-excision repair, DNA incision
phosphorylation of RNA polymerase II C-terminal domain
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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