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RAD51B Antibody (PA5-93318) in WB
Western blot analysis of RAD51B in extracts of various cell lines. Samples were incubated with RAD51B Polyclonal antibody (Product # PA5-93318) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93318
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-384 of human RAD51B (NP_5981932)
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806714
Product Specific Information
Immunogen sequence: MGSKKLKRVG LSQELCDRLS RHQILTCQDF LCLSPLELMK VTGLSYRGVH ELLCMVSRAC APKMQTAYGI KAQRSADFSP AFLSTTLSAL DEALHGGVAC GSLTEITGPP GCGKTQFCIM MSILATLPTN MGGLEGAVVY IDTESAFSAE RLVEIAESRF PRYFNTEEKL LLTSSKVHLY RELTCDEVLQ RIESLEEEII SKGIKLVILD SVASVVRKEF DAQLQGNLKE RNKFLAREAS SLKYLAEEFS IPVILTNQIT THLSGALASQ ADLVSPADDL SLSEGTSGSS CVIAALGNTW SHSVNTRLIL QYLDSERRQI LIAKSPLAPF TSFVYTIKEE GLVLQETTFC SVTQAELNWA PEILPPQPPE QLGLQMCHHT QLIF; Positive Samples: Mouse kidney, Rat kidney; Cellular Location: Nucleus
Target Information
Five human RAD 51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. Rad51B plays a role in meiotic recombination and/or recombinational repair.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein RAD51 homolog 2; hREC2; MGC34245; R51H2; R51H2REC2hREC2; RAD51 homolog B; RAD51 paralog B; RAD51-like 1; RAD51-like protein 1
Gene Aliases: AI553500; mREC2; R51H2; Rad51b; Rad51l1; Rec2; RGD1560681
UniProt ID: (Mouse) O35719
Entrez Gene ID: (Mouse) 19363, (Rat) 500679
double-strand break repair via homologous recombination
in utero embryonic development
blastocyst growth
DNA repair
DNA recombination
cellular response to DNA damage stimulus
positive regulation of cell proliferation
positive regulation of G2/M transition of mitotic cell cycle
somite development
DNA metabolic process
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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