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RAD51C Antibody (PA5-95324) in WB
Western blot analysis of Rad51C in, Lane 1: human 293T whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with RAD51C Polyclonal Antibody (Product # PA5-95324) at 0.5 μg/... View More
Product Details
PA5-95324
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Rad51C (177-207aa IQHLQLIAEKHKGEEHRKALEDFTLDNILSH).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807127
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ZFPM1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. ZFPM1 is an essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. ZFPM1 may be involved in regulation of some genes in gonads. ZFPM1 may also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein RAD51 homolog 3; MGC104277; R51H3; RAD51 homolog C; RAD51-like protein 2; yeast RAD51 homolog 3
Gene Aliases: BROVCA3; FANCO; R51H3; RAD51C; RAD51L2
UniProt ID: (Human) O43502
Entrez Gene ID: (Human) 5889
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA recombinase assembly
DNA synthesis involved in DNA repair
strand displacement
DNA repair
DNA recombination
mitotic recombination
sister chromatid cohesion
female meiosis sister chromatid cohesion
reciprocal meiotic recombination
male meiosis I
spermatogenesis
blood coagulation
response to ionizing radiation
positive regulation of G2/M transition of mitotic cell cycle
strand invasion
chromosome organization involved in meiotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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