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RAD54B Antibody (BS-5940R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RAD54B Polyclonal Antibody, Unconjugated (bs-5940R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-5940R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human RAD54B, amino acids 821-910.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair and recombination protein RAD54B; Fibrinogen silencer-binding protein; RAD54 homolog B; RAD54, S. cerevisiae, homolog of, B
Gene Aliases: E130016E03Rik; FSBP; RAD54B; RDH54; RGD1306507
UniProt ID: (Human) O95073, (Mouse) Q8BKE5
Entrez Gene ID: (Human) 25788, (Mouse) 623474, (Rat) 313063
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