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Invitrogen
RAE1 delta Monoclonal Antibody (RD-41), eBioscience™
FIGURE: 1 / 3
RAE1 delta Antibody (14-5756-81) in Flow
Staining of dendritic cell line with 0.125 µg of Mouse IgG1 kappa Isotype Control Purified (cat. 14-4714) (open histogram) or 0.125 µg of Anti-Mouse RAE1 delta Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE (cat. 12-4012). Total viable cells were used for analysis.
Product Details
14-5756-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RD-41
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1210509
Product Specific Information
Description: The RD-41 monoclonal antibody reacts with the murine Rae1delta. Rae-1delta is one of several known murine NKG2D ligands that include RAE-1 molecules (alpha, beta, ε, gamma and delta), H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands. The RD-41 antibody has been reported to block tetramer staining.
Applications Reported: This RD-41 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections and ELISA.
Applications Tested: This RD-41 antibody has been tested by flow cytometric analysis of dendritic cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rae-1 delta is one of several known murine NKG2D ligands that include RAE-1 molecules, H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: RAE-1-delta; retinoic acid early transcript 1D; Retinoic acid early-inducible protein 1-delta
Gene Aliases: RAE-1delta; Raet1d
UniProt ID: (Mouse) Q9JI58
Entrez Gene ID: (Mouse) 56554
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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