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Invitrogen
RAGE Polyclonal Antibody
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RAGE Antibody (PA5-48057) in WB
Western blot was performed using Anti-RAGE Polyclonal Antibody (Product # PA5-48057) and 45, 55 kDa bands corresponding to RAGE was observed in Mouse Lung, Rat Lung but not in Mouse Heart, Mouse Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Rat Lung (Lane 2), Mouse Heart (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB... View More
Product Details
PA5-48057
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Published species
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine RAGE Asp25-Val339
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576242
Product Specific Information
In sandwich ELISAs, approximately 0.5% cross-reactivity with recombinant human RAGE is observed and less than 0.1% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: advanced glycation end product receptor; RAGE-4 ORF3; RAGE/AGER; sRAGE
Gene Aliases: RAGE
Entrez Gene ID: (Dog) 403168
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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