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Invitrogen
RANBP1 Polyclonal Antibody
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RANBP1 Antibody (PA1-9093) in ICC/IF
Immunocytochemistry analysis of RANBP1 using RANBP1 Polyclonal Antibody (Product # PA1-9093) in paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA1-9093
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C terminus amino acids LSVKEETKEDAEEKQ (aa 187-201).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2176627
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HpaII tiny fragments locus 9A; Ran-binding protein 1; Ran-specific GTPase-activating protein; RanBP1; RANG; testis secretory sperm-binding protein Li 221n
Gene Aliases: HTF9A; RANBP1
UniProt ID: (Human) P43487
Entrez Gene ID: (Human) 5902
G1/S transition of mitotic cell cycle
RNA export from nucleus
ubiquitin-dependent protein catabolic process
protein import into nucleus
spindle organization
signal transduction
viral process
positive regulation of GTPase activity
positive regulation of mitotic centrosome separation
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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