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Invitrogen
RANBP1 Polyclonal Antibody
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FIGURE: 1 / 4
RANBP1 Antibody (PA5-21481) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RANBP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RANBP1 Polyclonal Antibody (Product # PA5-21481) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-21481
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RanBP1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152109
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain.
Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (84%), Pig (96%), Chicken (91%), Rhesus Monkey (99%), Bovine (88%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HpaII tiny fragments locus 9A; HpaII tiny fragments locus 9a protein; Htf9-a/RanBP1; Ran-binding protein 1; Ran-specific GTPase-activating protein; RANBP1; RANG; testis secretory sperm-binding protein Li 221n
Gene Aliases: Htf9-a; HTF9A; RANBP1
UniProt ID: (Human) P43487, (Mouse) P34022
Entrez Gene ID: (Human) 5902, (Mouse) 19385
G1/S transition of mitotic cell cycle
RNA export from nucleus
ubiquitin-dependent protein catabolic process
protein import into nucleus
spindle organization
signal transduction
viral process
positive regulation of GTPase activity
positive regulation of mitotic centrosome separation
cell-cell adhesion
positive regulation of neuron projection development
intracellular transport
cellular response to leptomycin B
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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