Proteintech

RANKL Polyclonal Antibody

RANKL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

RANKL Antibody (23408-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using (Product # 23408-1-AP) (RANKL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RANKL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

23408-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RANKL Fusion Protein Ag19975 (76-317 aa encoded by BC074890)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PNRISEDGT HCIYRILRLH ENADFQDTTL ESQDTKLIPD SCRRIKQAFQ GAVQKELQHI VGSQHIRAEK AMVDGSWLDL AKRSKLEAQP FAHLTINATD IPSGSHKVSL SSWYHDRGWA KISNMTFSNG KLIVNQDGFY YLYANICFRH HETSGDLATE YLQLMVYVTK TSIKIPSSHT LMKGGSTKYW SGNSEFHFYS INVGGFFKLR SGEEISIEVS NPSLLDPDQD ATYFGAFKVR DID (76-317 aa encoded by BC074890)

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11

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Gene Aliases: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788

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Entrez Gene ID: (Human) 8600

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Function(s)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RANKL Polyclonal Antibody

RANKL Antibody (23408-1-AP) in IHC (P)

Product Details

23408-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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