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Invitrogen
RAP1A Polyclonal Antibody
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RAP1A Antibody (PA5-87540) in IHC (P)
Immunohistochemistry analysis of RAP1A in paraffin-embedded human colon carcinoma. Samples were incubated with RAP1A Polyclonal antibody (Product # PA5-87540) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-87540
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 39-138 of human Rap1A (NP_0028751)
Conjugate
Form
Liquid
Concentration
0.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal, 0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804237
Product Specific Information
Positive Samples: U-87MG, HL-60, Mouse lung, Mouse kidney, Rat kidney, Rat brain; Cellular Location: Cell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region
Immunogen sequence: SYRKQVEVDC QQCMLEILDT AGTEQFTAMR DLYMKNGQGF ALVYSITAQS TFNDLQDLRE QILRVKDTED VPMILVGNKC DLEDERVVGK EQGQNLARQW
Target Information
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein SMG-P21A; Ras-related protein Krev-1; Ras-related protein Rap-1A
Gene Aliases: AI848598; C21KG; G-22K; KREV-1; KREV1; RAP1; RAP1A; SMGP21
UniProt ID: (Human) P62834, (Mouse) P62835
Entrez Gene ID: (Human) 5906, (Mouse) 109905
signal transduction
small GTPase mediated signal transduction
nervous system development
positive regulation of neuron projection development
protein transport
Rap protein signal transduction
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
regulation of cell junction assembly
cellular response to nerve growth factor stimulus
negative regulation of synaptic vesicle exocytosis
positive regulation of vasculogenesis
activation of MAPKK activity
microvillus assembly
negative regulation of collagen biosynthetic process
cellular response to drug
positive regulation of glucose import
positive regulation of phagocytosis
regulation of insulin secretion
cellular response to glucose stimulus
response to antineoplastic agent
liver regeneration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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