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Invitrogen
RAP1GAP Polyclonal Antibody
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RAP1GAP Antibody (PA5-106715) in IHC (P)
Immunohistochemistry analysis of RAP1GAP in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAP1GAP polyclonal antibody (Product # PA5-106715) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106715
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAP1GAP(Accession P47736), corresponding to amino acid residues S484-S534.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854383
Product Specific Information
Antibody detects endogenous levels of total RAP1GAP.
Target Information
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARPP-90; Rap1 GTPase-activating protein 1; Rap1, GTPase-activating protein 1; Rap1GAP
Gene Aliases: 1300019I11Rik; 2310004O14Rik; AI427470; ARPP-90; KIAA0474; RAP1GA1; RAP1GAP; RAP1GAP1; RAP1GAPII; RAPGAP
UniProt ID: (Human) P47736, (Mouse) A2ALS5
Entrez Gene ID: (Human) 5909, (Mouse) 110351, (Rat) 313644
negative regulation of cell proliferation
regulation of GTPase activity
positive regulation of GTPase activity
negative regulation of neuron differentiation
regulation of small GTPase mediated signal transduction
negative regulation of microvillus assembly
signal transduction
negative regulation of GTP binding
negative regulation of thyroid gland epithelial cell proliferation
cellular response to glial cell derived neurotrophic factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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