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RAP2C Antibody (PA5-101831) in ICC/IF
Immunocytochemistry analysis of RAP2C in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAP2C polyclonal antibody (Product # PA5-101831) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101831
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAP2C(Accession Q9Y3L5), corresponding to amino acid residues M96-S146.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851263
Product Specific Information
Antibody detects endogenous levels of total RAP2C.
Target Information
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ras-related protein Rap-2c; small GTPase RAP2C
Gene Aliases: 2010200P20Rik; AI194294; AL022976; RAP2C
UniProt ID: (Human) Q9Y3L5, (Mouse) Q8BU31
Entrez Gene ID: (Human) 57826, (Rat) 302495, (Mouse) 72065
microvillus assembly
negative regulation of cell migration
positive regulation of protein autophosphorylation
Rap protein signal transduction
regulation of protein tyrosine kinase activity
establishment of endothelial intestinal barrier
regulation of nucleic acid-templated transcription
signal transduction
small GTPase mediated signal transduction
Performance Guarantee
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