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Invitrogen
RAPGEF4 Polyclonal Antibody
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FIGURE: 1 / 2
RAPGEF4 Antibody (PA5-115183) in ICC/IF
Immunocytochemistry analysis of RAPGEF4 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115183) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115183
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAPGEF4(Accession Q8WZA2), corresponding to amino acid residues N944-D994.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899819
Product Specific Information
Antibody detects endogenous levels of total Epac2.
Target Information
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP-dependent Rap1 guanine-nucleotide exchange factor; cAMP-GEFII; cAMP-regulated guanine nucleotide exchange factor II; cAMP-regulated guanine nucleotide exchange factor IIB; EPAC 2; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; gefii; putative protein product of Nbla00496; Rap guanine nucleotide exchange factor (GEF) 4; Rap guanine nucleotide exchange factor 4; RAP guanine-nucleotide-exchange factor (GEF) 4
Gene Aliases: 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; CAMP-GEFII; CAMPS; CGEF2; EPAC; EPAC 2; Epac-2; EPAC2; mKIAA4040; Nbla00496; RAPGEF4
UniProt ID: (Human) Q8WZA2, (Mouse) Q9EQZ6
Entrez Gene ID: (Human) 11069, (Rat) 252857, (Mouse) 56508
G-protein coupled receptor signaling pathway
small GTPase mediated signal transduction
calcium ion regulated exocytosis
regulation of exocytosis
cAMP-mediated signaling
insulin secretion
positive regulation of GTPase activity
regulation of insulin secretion
exocytosis
signal transduction
heart development
positive regulation of smooth muscle cell migration
regulation of protein kinase activity
regulation of dendrite development
negative regulation of synaptic transmission
regulation of protein phosphorylation
intracellular signal transduction
positive regulation of action potential
positive regulation of protein secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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