OriGene

RARA Monoclonal Antibody (OTI2D5), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF806242

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D5

Immunogen

Human recombinant protein fragment corresponding to amino acids 52-299 of human RARA produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group B member 1; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RAR Alpha; RAR-alpha; retinoic acid; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; retinoic acid receptor; Retinoic acid receptor alpha; retinoic acid receptor, alpha polypeptide; RRA

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Gene Aliases: NR1B1; RAR; RARA

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UniProt ID: (Human) P10276

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Entrez Gene ID: (Human) 5914

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Function(s)
translation repressor activity, nucleic acid binding RNA polymerase II regulatory region sequence-specific DNA binding retinoic acid binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity transcription coactivator activity transcription corepressor activity receptor binding protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein domain specific binding chromatin DNA binding histone deacetylase binding protein kinase B binding retinoic acid-responsive element binding protein heterodimerization activity mRNA 5'-UTR binding protein kinase A binding alpha-actinin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development neural tube closure liver development glandular epithelial cell development outflow tract septum morphogenesis growth plate cartilage development transcription initiation from RNA polymerase II promoter protein phosphorylation signal transduction germ cell development spermatogenesis female pregnancy positive regulation of cell proliferation negative regulation of cell proliferation hippocampus development prostate gland development negative regulation of granulocyte differentiation embryonic camera-type eye development regulation of myelination response to estradiol response to retinoic acid negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production response to vitamin A response to cytokine multicellular organism growth negative regulation of apoptotic process apoptotic cell clearance steroid hormone mediated signaling pathway response to ethanol positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation regulation of synaptic plasticity retinoic acid receptor signaling pathway positive regulation of binding ventricular cardiac muscle cell differentiation Sertoli cell fate commitment limb development face development trachea cartilage development chondroblast differentiation negative regulation of cartilage development cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to estrogen stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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