Invitrogen

RARA Recombinant Rabbit Monoclonal Antibody (SN0725)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32325

Applications
Tested Dilution
Publications

Western Blot (WB)

1:50-1:2,000
-

Flow Cytometry (Flow)

1:50-1:100
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Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN0725

Immunogen

Synthetic peptide within Human Retinoic Acid Receptor alpha aa 1-50
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809606

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group B member 1; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RAR Alpha; RAR alpha 1; RAR-alpha; retinoic acid; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; retinoic acid receptor; Retinoic acid receptor alpha; retinoic acid receptor, alpha polypeptide; RRA

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Gene Aliases: NR1B1; RAR; RARA; RARalpha1

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UniProt ID: (Human) P10276, (Mouse) P11416

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Entrez Gene ID: (Human) 5914, (Mouse) 19401

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Function(s)
translation repressor activity, nucleic acid binding RNA polymerase II regulatory region sequence-specific DNA binding retinoic acid binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity transcription coactivator activity transcription corepressor activity receptor binding protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein domain specific binding chromatin DNA binding histone deacetylase binding protein kinase B binding retinoic acid-responsive element binding protein heterodimerization activity mRNA 5'-UTR binding protein kinase A binding alpha-actinin binding regulatory region DNA binding RNA polymerase II regulatory region DNA binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding phosphatidylinositol 3-kinase regulator activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development neural tube closure glandular epithelial cell development outflow tract septum morphogenesis growth plate cartilage development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation signal transduction germ cell development spermatogenesis positive regulation of cell proliferation negative regulation of cell proliferation male gonad development positive regulation of gene expression negative regulation of gene expression positive regulation of phosphatidylinositol 3-kinase signaling negative regulation of translation regulation of granulocyte differentiation negative regulation of granulocyte differentiation embryonic camera-type eye development regulation of myelination response to retinoic acid negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production multicellular organism growth regulation of apoptotic process negative regulation of apoptotic process apoptotic cell clearance steroid hormone mediated signaling pathway regulation of phosphatidylinositol 3-kinase activity response to ethanol negative regulation of cell differentiation positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation regulation of synaptic plasticity retinoic acid receptor signaling pathway positive regulation of binding positive regulation of protein kinase B signaling ventricular cardiac muscle cell differentiation Sertoli cell fate commitment limb development face development bone development trachea cartilage development chondroblast differentiation negative regulation of cartilage development positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to estrogen stimulus liver development transcription initiation from RNA polymerase II promoter female pregnancy hippocampus development prostate gland development response to estradiol response to vitamin A response to cytokine
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