Invitrogen

RARB Monoclonal Antibody (R beta 28)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-811

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250
-

ELISA (ELISA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fish, Mouse, Non-human primate, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

R beta 28

Immunogen

Synthetic peptide corresponding to the C-terminal sequence of the mouse RAR beta.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2177762

Product Specific Information

MA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues. This antibody cross-reacts slightly with RAR alpha when expressed in COS cells, though the level of signal on Western blots is very low relative to that seen with RAR beta. It does not detect RAR alpha or gamma from cell lines or tissue extract.

MA1-811 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~52 kDa protein in rat tissue extract representing RAR beta. Also, by Western blot MA1-811 detects an unidentified ~120 kDa protein which can be competed away with the immunizing peptide. This protein is most notable in human SH-SY5Y and COS cells. Due to the extremely low levels of RAR expressed in native tissues, it is recommended that sensitive detection systems, such as enhanced chemiluminescence, be used.

The MA1-811 immunogen is a synthetic peptide corresponding to the C-terminal sequence of mouse RAR beta.

Reconstitute with PBS.

MA1-811 can be used with blocking peptide PEP-005.

Target Information

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HBV-activated protein; hepatitis B virus activated protein; Nuclear receptor subfamily 1 group B member 2; RAR beta; RAR-b; RAR-beta; RAR-Beta2; RAR-Beta4; RAR-epsilon; retinoic acid; retinoic acid receptor; Retinoic acid receptor beta; retinoic acid receptor, beta polypeptide

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Gene Aliases: A830025K23; HAP; MCOPS12; NR1B2; RARB; RARbeta1; RRB2

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UniProt ID: (Human) P10826, (Mouse) P22605

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Entrez Gene ID: (Human) 5915, (Rat) 24706, (Mouse) 218772

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding DNA binding steroid hormone receptor activity retinoic acid receptor activity drug binding zinc ion binding protein complex binding retinoid X receptor binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development glandular epithelial cell development outflow tract septum morphogenesis retinal pigment epithelium development growth plate cartilage development transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cell proliferation negative regulation of cell proliferation striatum development neurogenesis regulation of myelination negative regulation of chondrocyte differentiation embryonic hindlimb morphogenesis multicellular organism growth positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death steroid hormone mediated signaling pathway positive regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter embryonic eye morphogenesis retinoic acid receptor signaling pathway embryonic digestive tract development ventricular cardiac muscle cell differentiation retina development in camera-type eye bone development negative regulation of cartilage development transcription initiation from RNA polymerase II promoter signal transduction regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter
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