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Invitrogen
RASA1 Monoclonal Antibody (B4F8)
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FIGURE: 1 / 5
RASA1 Antibody (MA4-001) in ICC/IF
Immunofluorescent analysis of GTPase Activating Protein (green) showing positive staining in the cytoplasm of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a GTPase Activating Protein monoclonal antibody (Product # MA4-001) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were was... View More
Product Details
MA4-001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
B4F8
Immunogen
Full length recombinant human GAP.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2175873
Product Specific Information
MA4-001 detects GTPase activating protein (GAP) from human, mouse, rat, bovine and non-human primate tissues.
MA4-001 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. Immunofluorescence staining of GAP in mouse fibroblast cells with MA4-001 results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. MA4-001 immunoprecipitates GAP that is complexed with the GAP related p190.
The MA4-001 antigen is full length recombinant human GAP. Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 & SH3.
Reconstitute with PBS.
Target Information
TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 4,8, and 10. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GAP; GTPase-activating protein; p120 RAS GTPase activating protein; p120-rasGAP; p120GAP; Ras GTPase-activating protein 1; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein RAS p21); RAS p21 protein activator (GTPase activating protein) 1; RAS p21 protein activator 1; rasGAP; triphosphatase-activating protein
Gene Aliases: BOS_8464; CM-AVM; CMAVM; GAP; GAPX; p120; p120GAP; p120RASGAP; PKWS; RASA; RASA1; RASGAP
UniProt ID: (Human) P20936, (Bovine) P09851, (Rat) P50904
Entrez Gene ID: (Human) 5921, (Bovine) 282032, (Rat) 25676, (Mouse) 218397
glycoprotein binding
GTPase activator activity
receptor binding
protein binding
potassium channel inhibitor activity
GTPase binding
phospholipid binding
platelet-derived growth factor receptor binding
kinase binding
protein kinase binding
receptor tyrosine kinase binding
protein complex binding
ion channel binding
MAPK cascade
mitotic cytokinesis
vasculogenesis
negative regulation of cell-matrix adhesion
negative regulation of cell adhesion
signal transduction
regulation of cell shape
embryo development
regulation of actin filament polymerization
intracellular signal transduction
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
negative regulation of Ras protein signal transduction
ephrin receptor signaling pathway
blood vessel morphogenesis
regulation of RNA metabolic process
cell adhesion
actin cytoskeleton organization
positive regulation of protein phosphorylation
cellular response to platelet-derived growth factor stimulus
positive regulation of glucose import
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
cellular response to epidermal growth factor stimulus
activation of GTPase activity
cytokinesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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