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RASGRF1 Antibody (PA5-93320) in WB
Western blot analysis of RASGRF1 in mouse brain. Samples were incubated with RASGRF1 Polyclonal antibody (Product # PA5-93320) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93320
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_0011391201)
Conjugate
Form
Liquid
Concentration
2.39 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806715
Product Specific Information
Immunogen sequence: MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS; Positive Samples: Mouse brain
Target Information
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASPP1; CDC25Mm; GNRP; Guanine nucleotide-releasing protein; KIAA0771; PPP1R13B; Ras guanine release factor beta; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25
Gene Aliases: AI844718; CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190; P190-A; p190RhoGEF; Ras-GRF1; Rasgrf1
UniProt ID: (Mouse) P27671
Entrez Gene ID: (Mouse) 19417
small GTPase mediated signal transduction
cell proliferation
neuron projection development
regulation of Rac protein signal transduction
regulation of Rho protein signal transduction
intracellular signal transduction
positive regulation of GTPase activity
regulation of Ras protein signal transduction
positive regulation of Ras protein signal transduction
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
activation of GTPase activity
regulation of N-methyl-D-aspartate selective glutamate receptor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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