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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Rb Antibodies

          Proteintech

          RB1 Polyclonal Antibody

          55 References
          View all (119) Rb antibodies

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          Datasheet
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          Datasheet
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          Cite RB1 Polyclonal Antibody

          • Antibody Testing Data (9)
          RB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          RB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          RB1 Antibody (10048-2-IG) in IHC (P)

          Immunohistochemistry of paraffin-embedded human prostate cancer using 10048-2-IG (RB1 antibody) at dilution of 1:200 (under 10x lens). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RB1 Antibody in Western Blot (WB)
          RB1 Antibody in Western Blot (WB)
          RB1 Antibody in Western Blot (WB)
          RB1 Antibody in Western Blot (WB)
          RB1 Antibody in Western Blot (WB)
          RB1 Antibody in Immunoprecipitation (IP)

          Product Details

          10048-2-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:6,000
          View 40 publications 40 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          RB1 Fusion Protein Ag0006

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.

          Target Information

          Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp105; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

          View more View less

          Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; Rb-1; RB1

          View more View less

          UniProt ID: (Human) P06400, (Mouse) P13405

          View more View less

          Entrez Gene ID: (Human) 5925, (Mouse) 19645

          View more View less

          Function(s)
          core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding enzyme binding
          Process(es)
          cell cycle checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle chromatin remodeling transcription, DNA-templated negative regulation of protein kinase activity cell cycle arrest negative regulation of transcription from RNA polymerase II promoter during mitosis mitotic cell cycle checkpoint Ras protein signal transduction regulation of mitotic cell cycle negative regulation of gene expression viral process covalent chromatin modification androgen receptor signaling pathway sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell cycle negative regulation of cell proliferation chromatin modification neuron differentiation negative regulation of cell cycle negative regulation of mitotic cell cycle regulation of cell cycle negative regulation of hepatocyte apoptotic process
          It has to be done as per old AB suggested Products section.
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