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RBM25/RED120 Antibody (A301-068A) in IHC (P)
Detection of human and mouse RBM25/RED120 by immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti-RBM25/RED120 (Product # A301-068A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
Product Details
A301-068A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 475 and 525 of Human RNA binding motif protein 25 (Arg/Glu/Asp-rich protein, 120 kDa).
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arg/Glu/Asp-rich protein of 120 kDa; Arg/Glu/Asp-rich protein, 120 kDa; functional spliceosome-associated protein 94; Protein S164; RED120; rna-binding; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding region (RNP1, RRM) containing 7; RNA-binding region-containing protein 7; U1 small nuclear ribonucleoprotein 1SNRP homolog
Gene Aliases: 2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; fSAP94; NET52; RBM25; RED120; RNPC7; S164; Snu71
UniProt ID: (Human) P49756, (Mouse) B2RY56
Entrez Gene ID: (Human) 58517, (Mouse) 67039
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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