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RBM5 Antibody (PA5-88039) in WB
Western blot analysis of RBM5 in extracts of various cell lines. Samples were incubated with RBM5 Polyclonal antibody (Product # PA5-88039) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88039
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBM5 (NP_0057691)
Conjugate
Form
Liquid
Concentration
2.55 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804609
Product Specific Information
Immunogen sequence: MGSDKRVSRT ERSGRYGSII DRDDRDERES RSRRRDSDYK RSSDDRRGDR YDDYRDYDSP ERERERRNSD RSEDGYHSDG DYGEHDYRHD ISDERESKTI MLRGLPITIT ESDIREMMES FEGPQPADVR; Positive Samples: U-87MG, 293T, HeLa, LO2, A-549, Mouse thymus, Mouse liver, Rat thymus; Cellular Location: Nucleus
Target Information
DEF-3 and LUCA15 belong to an evolutionarily conserved family of RNA binding proteins and share similar expression patterns. Both DEF-3 and LUCA15 are highly expressed in adult heart and thymus as well as fetal kidney. Conversely, fetal thymus and adult kidney express very little DEF-3 and LUCA15. In the haemopoietic system of mice, the expression of DEF-3 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development. Both DEF-3 and LUCA15 contain two zinc finger motifs, a bipartite nuclear signal and two RNA binding motifs. DEF-3 and LUCA15 are capable of specifically binding poly(G) RNA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein G15; Putative tumor suppressor LUCA15; Renal carcinoma antigen NY-REN-9; RNA-binding motif protein 5; RNA-binding protein 5
Gene Aliases: D030069N10Rik; G15; H37; LUCA15; RBM5; RMB5
UniProt ID: (Human) P52756, (Mouse) Q91YE7, (Rat) B2GV05
Entrez Gene ID: (Human) 10181, (Mouse) 83486, (Rat) 300996
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