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RBMS3 Antibody (PA5-114400) in ICC/IF
Immunocytochemistry analysis of RBMS3 in U20S cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in RBMS3 polyclonal antibody (Product # PA5-114400) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
PA5-114400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human RBMS3 recombinant protein (Position: T350-P437).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884856
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U2OS whole cell, human PC-3 whole cell, human U-87MG whole cell, human A549 whole cell, human Hela whole cell, rat heart tissue, mouse testicular tissue. IHC: human oesophagus squama cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes an RNA-binding protein that belongs to the c-myc gene single-strand binding protein family. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The encoded protein was isolated by virtue of its binding to an upstream element of the alpha2(I) collagen promoter. The observation that this protein localizes mostly in the cytoplasm suggests that it may be involved in a cytoplasmic function such as controlling RNA metabolism, rather than transcription. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA binding motif, single stranded interacting protein 3; RNA-binding motif, single-stranded-interacting protein 3; RNA-binding protein; RNA-binding protein RBMS3
Gene Aliases: 6720477E09Rik; 8430436O14Rik; RBMS3
UniProt ID: (Human) Q6XE24, (Mouse) Q8BWL5
Entrez Gene ID: (Human) 27303, (Mouse) 207181, (Rat) 680726
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