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FIGURE: 1 / 1
RBMX Antibody (MA5-51303) in WB
Western blot analysis of RBMX in various lysates. Samples were incubated with RBMX Recombinant monoclonal antibody (Product # MA5-51303) using a dilution of 1:20,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-51303
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7N9Z5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 292-391 of human RBMX/hnRNP G (NP_002130.2).
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094243
Product Specific Information
Immunogen sequence: RSAPPTRGPP PSYGGSSRYD DYSSSRDGYG GSRDSYSSSR SDLYSSGRDR VGRQERGLPP SMERGYPPPR DSYSSSSRGA PRGGGRGGSR SDRGGGRSRY
Target Information
hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G; RNA binding motif protein, X chromosome; RNA-binding motif protein, X chromosome; RNA-binding motif protein, X chromosome retrogene; RNA-binding motif protein, X chromosome retrogene-like
Gene Aliases: hnRNP G; hnRNP-G; HNRNPG; HNRPG; MRXS11; RBMX; RBMXP1; RBMXRT; RNMX
UniProt ID: (Human) Q5JQ67, (Mouse) Q9WV02, (Rat) Q4V898
Entrez Gene ID: (Human) 27316, (Mouse) 19655, (Rat) 302855
regulation of alternative mRNA splicing, via spliceosome
osteoblast differentiation
transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA splice site selection
mRNA processing
membrane protein ectodomain proteolysis
RNA splicing
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to interleukin-1
mRNA splicing, via spliceosome
gene expression
negative regulation of mRNA splicing, via spliceosome
positive regulation of mRNA splicing, via spliceosome
protein homooligomerization
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