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RCC2 Antibody (A300-872A) in WB
Detection of human and mouse RCC2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-RCC2 antibody BL3701 (Product # A300-872A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Product Details
A300-872A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human Regulator of Chromosome Condensation 2.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa
Gene Aliases: 2610510H01Rik; 2610529N02Rik; AA536646; AA675016; KIAA1470; mKIAA1470; RCC2; TD-60; TD60
UniProt ID: (Human) Q9P258, (Mouse) Q8BK67
Entrez Gene ID: (Human) 55920, (Mouse) 108911
sister chromatid cohesion
mitotic nuclear division
integrin-mediated signaling pathway
regulation of fibroblast migration
positive regulation of G2/M transition of mitotic cell cycle
regulation of cell migration
negative regulation of GTPase activity
establishment of protein localization
focal adhesion assembly
cell division
negative regulation of focal adhesion assembly
positive regulation of attachment of spindle microtubules to kinetochore
chromosome passenger complex localization to kinetochore
activation of GTPase activity
negative regulation of substrate adhesion-dependent cell spreading
regulation of ruffle assembly
mitotic cell cycle
endosome organization
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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