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Invitrogen
REA Monoclonal Antibody (2B5)
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REA Antibody (MA5-49251) in IHC (P)
Immunohistochemistry analysis of REA in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with REA Monoclonal antibody (Product # MA5-49251) using a dilution of 2 μg/mL overnight at 4°C. Biotinylated goat anti-mou... View More
Product Details
MA5-49251
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2B5
Immunogen
E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074909
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, rat brain tissue, rat heart tissue, rat liver tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue. IHC: human gallbladder adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue, human renal clear cell carcinoma tissue, human lung cancer tissue, mouse colon tissue, rat colon tissue. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. This protein functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. It is probably involved in regulating mitochondrial respiration activity and in aging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell associated protein; B-cell receptor associated protein 37; B-cell receptor-associated protein 37; B-cell receptor-associated protein BAP37; BAP-37; D-prohibitin; MGC117268; PHB2; phb2 {ECO:0000250|UniProtKB:Q99623}; Prohibitin-2; Repressor of estrogen receptor activity
Gene Aliases: AU044498; BAP; Bap-37; Bap37; Bcap27; BCAP37; hBAP; p22; PHB2; PNAS-141; REA
UniProt ID: (Human) Q99623, (Mouse) O35129, (Rat) Q5XIH7
Entrez Gene ID: (Human) 11331, (Mouse) 12034, (Rat) 114766
protein import into nucleus, translocation
transcription, DNA-templated
regulation of transcription, DNA-templated
mitochondrion organization
sister chromatid cohesion
positive regulation of exit from mitosis
negative regulation of intracellular estrogen receptor signaling pathway
negative regulation of mammary gland epithelial cell proliferation
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
protein stabilization
positive regulation of sequence-specific DNA binding transcription factor activity
mammary gland branching involved in thelarche
mammary gland alveolus development
regulation of branching involved in mammary gland duct morphogenesis
positive regulation of ERK1 and ERK2 cascade
positive regulation of cell cycle G1/S phase transition
regulation of complement activation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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