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Invitrogen
REC8 Polyclonal Antibody
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FIGURE: 1 / 1
REC8 Antibody (PA5-120322) in WB
Western blot analysis of REC8 in extracts of Rat testis. Samples were incubated with REC8 Polyclonal antibody (Product # PA5-120322) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120322
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 194-561 of mouse REC8 (NP_064386.2).
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913894
Product Specific Information
Positive test controls include: Rat testis. The target is usually found in the following locations: Chromosome, Nucleus.
Immunogen sequence: EVITLQEAEP IRMLQIEGEQ DLPEISRGDL ELLIAEKDDA ILLEERQRGR LLRQRRASLP LDESREEPRA LEGAGLVSAL SPPAPAQVEG IQEALPGQVF PPEVQKMTGW EPGALLTEVT PPQELRLPAP PSTEKRLPSL QRPLPRRHRR RQLLFWDKET QISREKFEEQ LQTGAHCWEY PVAQPPKRML TSPAELFRTP TLSGWLPPEL LGLWTHCAQV PQRMLRQRPQ LETEETVEEE RAADEEERRK TEALSEIEVL REAQEPSGPL MLSSELSLEA AEDEKSRTSL IPPEWWAWSE EGQPEPPALP MLPELPEVPM EMPPRPELSS EAVLRAVALK LQANKELDFS SLVPPLSPRK LASRVFYL
Target Information
This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008].
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cohesin Rec8p; meiosis specific sister chromatid cohesion protein; Meiotic recombination protein REC8 homolog; REC8 homolog; rec8 {ECO:0000250|UniProtKB:Q8C5S7}; REC8-like 1
Gene Aliases: Rec8; Rec8L1
UniProt ID: (Rat) Q6AYJ4
Entrez Gene ID: (Rat) 290227
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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