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RELM alpha Antibody (500-P214BT-25UG) in WB
Western Blot: To detect Murine RELMalpha by Western Blot analysis RELM alpha Polyclonal Antibody, Biotin (Product # 500-P214BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P214BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 10.0 kDa Recombinant Murine RELMalpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929853
Product Specific Information
AA Sequence of recombinant protein: MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMalpha. Anti-Murine RELMalpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine RELMalpha (500-P214) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMalpha.
Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P214BT-1MG will be provided as 2 x 500 µg
Target Information
RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; Hypoxia-induced mitogenic factor; Parasite-induced macrophage novel gene 1 protein; RELM-beta; RELMalpha; RELMb; RELMbeta; Resistin-like alpha
Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; Pmng1; RELM-alpha; RELMa; RELMalpha; Retnla; Xcp2
UniProt ID: (Mouse) Q9EP95
Entrez Gene ID: (Mouse) 57262
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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