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Invitrogen
RELM alpha Monoclonal Antibody (226033)
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FIGURE: 1 / 1
RELM alpha Antibody (MA5-23898) in ICC/IF
Immunocytochemistry analysis of RELM alpha in immersion fixed BV-2 mouse microglia cell line. Samples were incubated in RELM alpha monoclonal antibody (Product # MA5-23898) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
MA5-23898
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
226033
Immunogen
E. coli-derived recombinant mouse RELM alpha Asp24-Ser111
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605577
Product Specific Information
In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) RELM beta or rmResistin.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; Hypoxia-induced mitogenic factor; Parasite-induced macrophage novel gene 1 protein; RELM-beta; RELMalpha; RELMb; RELMbeta; Resistin-like alpha
Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; Pmng1; RELM-alpha; RELMa; RELMalpha; Retnla; Xcp2
UniProt ID: (Mouse) Q9EP95
Entrez Gene ID: (Mouse) 57262
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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