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RELM beta Antibody (500-P215-1MG) in WB
Western Blot: To detect Murine RELMbeta by Western Blot analysis RELM beta Polyclonal Antibody (Product # 500-P215-1MG) can be used at a concentration of 0.1-0.2 µg/mL. when used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMbeta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P215-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine RELMbeta
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929856
Product Specific Information
AA Sequence of recombinant protein: MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMbeta. Anti-Murine RELMbeta-specific antibody was purified by affinity chromatography employing an immobilized Murine RELMbeta matrix.
Sandwich ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RELMbeta (500-P215Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMbeta.
Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. when used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMbeta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Adipose tissue is an energy reserve that is strictly regulated in animals. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cysteine-rich secreted protein A12-beta; Cysteine-rich secreted protein FIZZ2; found in inflammatory zone 2; RELMbeta; Resistin-like beta; resistin-like molecule beta; RSNB
Gene Aliases: 9030012B21Rik; Fizz2; Relmb; RELMbeta; Retnlb; Xcp3
UniProt ID: (Mouse) Q99P86
Entrez Gene ID: (Mouse) 57263
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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