Invitrogen

REST Recombinant Rabbit Monoclonal Antibody (JE32-25)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-44189

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE32-25

Immunogen

Recombinant protein within Human REST aa 850-1000
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2926319

Product Specific Information

Predicted band size: 122 kDa

Positive Control: HL-60 cell lysate, HUVEC cell lysate, human thyroid tissue, human seminoma tissue, SH-SY5Y.

Target Information

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Neural-restrictive silencer factor; neuron restrictive silencer factor; neuron-restrictive silencer factor; RE1-silencing transcription factor; RE1-silencing transcription factor variant E1a/E2/E3/E4c; RE1-silencing transcription factor variant E1a/E2/E3/E5; RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i; RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1a/E2/E4; RE1-silencing transcription factor variant E1a/E2/E5; RE1-silencing transcription factor variant E1a/E2a/E2k; RE1-silencing transcription factor variant E1a/E2d/E4g; RE1-silencing transcription factor variant E1a/E2e/E4h; RE1-silencing transcription factor variant E1a/E2f/E4e; RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j; RE1-silencing transcription factor variant E1b/E2/E3/E5; RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i; RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1b/E2a/E2k; RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4; RE1-silencing transcription factor variant E1b/E2e/E4h; RE1-silencing transcription factor variant E1c/E2/E3/E5; RE1-silencing transcription factor variant E1c/E2a/E2k; RE1-silencing transcription factor variant E1c/E2g/E3/E4; repressor binding to the X2 box; X2 box repressor

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Gene Aliases: NRSF; REST; WT6; XBR

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UniProt ID: (Human) Q13127

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Entrez Gene ID: (Human) 5978

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Function(s)
core promoter sequence-specific DNA binding core promoter binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding outward rectifier potassium channel activity protein complex binding transcription regulatory region DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter hematopoietic progenitor cell differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter negative regulation of cell proliferation negative regulation of gene expression negative regulation of aldosterone biosynthetic process cellular response to drug positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation by host of viral transcription negative regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis negative regulation of insulin secretion negative regulation of neurogenesis cardiac muscle cell myoblast differentiation histone H4 deacetylation cellular response to electrical stimulus cellular response to glucocorticoid stimulus potassium ion transmembrane transport negative regulation of cortisol biosynthetic process negative regulation of dense core granule biogenesis negative regulation of mesenchymal stem cell differentiation negative regulation of amniotic stem cell differentiation
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