Invitrogen

RETSAT Polyclonal Antibody

5 Published Figures

6 References

View all (3) RETSAT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (5)

RETSAT Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

RETSAT Antibody (PA5-65443) in ICC/IF

Immunofluorescent staining of RETSAT in human cell line U-251 MG shows localization to nucleoli. Samples were probed using a RETSAT Polyclonal Antibody (Product # PA5-65443).

RETSAT Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

RETSAT Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RETSAT Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA5-65443

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human RETSAT. Recombinant protein control fragment (Product #RP-104822).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2663683

Product Specific Information

Immunogen sequence: SSPNPFSEDVK RPPAPLVTDK EARKKVLKQA FSANQVPEKL DVVVIGSGFG GLAAAAILAK AGKRVLVLEQ HTKAGGCCHT FGKNGLEFDT GIHYIGRMEE GSIGRFILDQ ITEGQLDWAP LSSPFDIMVL EGPNGRKEYP M

Highest antigen sequence identity to the following orthologs - mouse 88%, rat 89%.

Target Information

Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

PA5-65443 was used in Western Blotting to pave the way for personalized medicine by identifying potential biomarkers for ATR inhibitor resistance.

Sun Nov 01 00:00:00 UTC 2020

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

PA5-65443 was used in Western Blotting to pave the way for personalized medicine by identifying potential biomarkers for ATR inhibitor resistance.

Sun Nov 01 00:00:00 UTC 2020

A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells.

mBio

Chidawanyika T,Mark KMK,Supattapone S

PA5-65443 was used in Western Blotting to show that vitamin playing a role in controlling transport of a small molecule across the cell membrane.

Tue Aug 11 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: All-trans-13,14-dihydroretinol saturase; All-trans-retinol 13,14-reductase; PPAR-alpha-regulated and starvation-induced gene protein; retinol saturase (all-trans-retinol 13,14-reductase); RetSat

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Gene Aliases: PPSIG; RETSAT; UNQ439/PRO872

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UniProt ID: (Human) Q6NUM9

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Entrez Gene ID: (Human) 54884

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Function(s)

oxidoreductase activity all-trans-retinol 13,14-reductase activity

Process(es)

retinol metabolic process oxidation-reduction process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RETSAT Polyclonal Antibody

RETSAT Antibody (PA5-65443) in ICC/IF

Product Details

PA5-65443

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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