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Invitrogen
REV1 Polyclonal Antibody
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FIGURE: 1 / 2
REV1 Antibody (PA5-100870) in ICC/IF
Immunocytochemistry analysis of REV1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with REV1 polyclonal antibody (Product # PA5-100870) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human REV1(Accession Q9UBZ9), corresponding to amino acid residues Q1219-T1251.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850367
Product Specific Information
Antibody detects endogenous levels of total REV1.
Target Information
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIBP80; Alpha integrin-binding protein 80; DNA repair protein REV1; REV1 homolog; REV1, polymerase (DNA directed); REV1- like; Rev1-like terminal deoxycytidyl transferase
Gene Aliases: 1110027I23Rik; AIBP80; AU022044; REV1; REV1L
UniProt ID: (Human) Q9UBZ9, (Mouse) Q920Q2
Entrez Gene ID: (Human) 51455, (Mouse) 56210
damaged DNA binding
protein binding
deoxycytidyl transferase activity
metal ion binding
magnesium ion binding
DNA binding
telomerase activity
RNA helicase activity
DNA-directed DNA polymerase activity
DNA primase activity
sulfate adenylyltransferase activity
RNA guanylyltransferase activity
tRNA guanylyltransferase activity
mannose-phosphate guanylyltransferase activity
transferase activity
nucleotidyltransferase activity
DNA/RNA helicase activity
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity
phospholactate guanylyltransferase activity
ATP:coenzyme F420 adenylyltransferase activity
UDP-N-acetylgalactosamine diphosphorylase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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