Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Rockland
RFA2 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
RFA2 Antibody (600-401-448) in WB
This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive Lot 13229 at 1.0 µg/ml followed by 1:5000 dilution of IRDye800 Sheep anti Rabbit IgG (lot 13218) for 1 hr at RT.
Product Details
600-401-448
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2).
Conjugate
Form
Liquid
Concentration
0.12 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against yeast RFA2. This antibody consists of antibodies eluted from the non-phospho peptide column.
Target Information
Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DNA-binding protein BUF1; Replication factor A protein 2; Replication protein A 36 kDa subunit; RF-A protein 2; Rfa2p
Gene Aliases: BUF1; N0368; RFA2; RPA2; YNL312W
UniProt ID: (Baker's yeast) P26754
Entrez Gene ID: (Baker's yeast) 855404
telomere maintenance via recombination
double-strand break repair via homologous recombination
DNA replication
DNA unwinding involved in DNA replication
DNA repair
nucleotide-excision repair
mitotic recombination
telomere maintenance via telomerase
reciprocal meiotic recombination
protein ubiquitination
heteroduplex formation
establishment of protein localization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support