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Invitrogen
RFC1 Polyclonal Antibody
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FIGURE: 1 / 5
RFC1 Antibody (PA5-79923) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of RFC1 in U2OS cell using RFC1 Polyclonal Antibody (Product # PA5-79923). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight 488 conjugated goat anti-rabbit IgG and DyLight 594 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C.... View More
Product Details
PA5-79923
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1 (46-64aa NSSRKEDDFKQKQPSKKKR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747038
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human Hela whole cell, human HepG2 whole cell, human Raji whole cell, human K562 whole cell, human CACO-2 whole cell. IHC: human mammary cancer tissue. ICC/IF: U2OS cell. Flow: Hela cell.
Target Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A1 140 kDa subunit; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; MHC binding factor, beta; replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; Replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit
Gene Aliases: A1; MHCBFB; PO-GA; RECC1; RFC; RFC1; RFC140
UniProt ID: (Human) P35251
Entrez Gene ID: (Human) 5981
telomere maintenance via recombination
DNA-dependent DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
transcription, DNA-templated
regulation of transcription, DNA-templated
telomere maintenance via telomerase
translesion synthesis
nucleotide-excision repair, DNA incision
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of catalytic activity
error-free translesion synthesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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